Figure 6 Figure 6. . Genotypic characterization of

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Figure 6 Figure 6. . Genotypic characterization of
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Figure 6 Figure 6. . Genotypic characterization of wild-type flagellates by PCR amplification with rDNA and mini-exon specific primers derived from Trypanosoma cruzi. A, template DNAs amplified with mini-exon intergenic spacer primers (38):

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Figure 6 Figure 6. . Genotypic characterization of wild-type flagellates by PCR amplification with rDNA and mini-exon specific primers derived from Trypanosoma cruzi. A, template DNAs amplified with mini-exon intergenic spacer primers (38): Blank, negative control; Tcb, archetypic type II T. cruzi Berenice; Rp1, Dm1, Dm2 and Dm3, flagellates isolated from Rhodnius pictipes and from Didelphis marsupialis; Dm28, standard type I, sylvatic T. cruzi isolate. B, same template DNAs amplified with rDNA primers (39-41). Tcb yielded typical 300-bp band of type II lineage, whereas Rp1, Dm1, Dm2, and Dm3 and Dm28 yielded a 350-bp band of type I T. cruzi lineage, with mini-exon spacer primers. In addition, Tcb yielded typical a 125-bp band of type II, whereas the sylvatic T. cruzi isolates yielded a 110-bp band of type I, with rDNA primers. These findings confirm sylvatic Rp1, Dm1, Dm2, and Dm3 as T. cruzi. Teixeira A, Monteiro P, Rebelo JM, Argañaraz ER, Vieira D, Lauria-Pires L, et al. Emerging Chagas Disease: Trophic Network and Cycle of Transmission of Trypanosoma cruzi from Palm Trees in the Amazon. Emerg Infect Dis. 2001;7(1):100-112. https://doi.org/10.3201/eid0701.070100<br>