PPT-Figure A1 Figure A1. . Representative results of groEL heminested polymerase chain reaction

Alberti A Addis M Sparagano O Zobba R Chessa B Cubeddu T et al Anaplasma phagocytophilum Sardinia Italy Emerg Infect Dis 200511813221324 httpsdoiorg103201eid1108050085. (PCR). PCR. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone). Allows scientists to isolate pure quantities of specific DNA sequences. . The . first . reaction (. A). . to yield . 2. proceeded via a radical mechanism with commercially available . vinyltrimethylsilane. , . 1. . After . careful workup and purification, the product was determined via . by: Keeanna Wolcik and Gabbie Hahn. Key Terms. denature ~ in the DNA sense, unwind. anneal ~ bind. primers ~ strand of nucleic acids that are used as a starting point for DNA synthesis. Taq polymerase ~ an enzyme that synthesizes polymers; from bacteria that live in hot springs; . Nahla . Bakhamis. Multiple copies of specific DNA . sequences;. . ‘Molecular Photocopying’ . Polymerase Chain Reaction. 1983;. In . vitro. enzymatic amplification of specific DNA sequences from . dopamine D4 receptors. Pegi Pavletić. . 1,. *, F. abio. Del . Bello. . 1. , Alessandro Piergentili . 1. , and . Wilma. . Quaglia. . 1. 1. 1. School of Pharmacy, Medicinal Chemistry Unit, . University of . Kochar DK, Saxena V, Singh N, Kochar SK, Kumar SV, Das A. Plasmodium vivax Malaria. Emerg Infect Dis. 2005;11(1):132-134. https://doi.org/10.3201/eid1101.040519. Morré SA, Spaargaren J, Fennema J, de Vries H, Coutinho RA, Peña A. Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum. Emerg Infect Dis. 2005;11(8):1311-1312. https://doi.org/10.3201/eid1108.050535. Figure 6.01. Polymerase Chain Reaction (PCR). During PCR, two primers anneal to complementary sequences at either end of a target sequence on a piece of denatured sample DNA. . DNA polymerase. synthesizes a complementary strand of DNA from the primers, resulting in two new strands of DNA. In further cycles, the newly made DNA molecules are denatured, primers are annealed, and DNA polymerase copies the target regions, resulting in multiple copies of the original target sequence.. Figure 8.01. Sequencing—Fragments of All Possible Lengths. During chain termination or . Sanger sequencing. , the target DNA fragments are copied millions of times, but each copy ends at a different nucleotide position. These subsets of fragments end with a fluorescently-labeled nucleotide that reveal the identity of the final base. The final sequencing data are a series of fluorescent peaks that correspond to the original template . Ranque S, Faugère B, Pozio E, La Rosa G, Tamburrini A, Pellissier J, et al. Trichinella pseudospiralis Outbreak in France. Emerg Infect Dis. 2000;6(5):543-547. https://doi.org/10.3201/eid0605.000517. Marcus PI, Girshick T, van der Heide L, Sekellick MJ. Super-Sentinel Chickens and Detection of Low-Pathogenicity Influenza Virus. Emerg Infect Dis. 2007;13(10):1608-1610. https://doi.org/10.3201/eid1310.061552. Johnson DJ, Ostlund EN, Pedersen DD, Schmitt BJ. Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay. Emerg Infect Dis. 2001;7(4):739-741. https://doi.org/10.3201/eid0704.017425. Cama V, Gilman RH, Vivar A, Ticona E, Ortega Y, Bern C, et al. Mixed Cryptosporidium Infections and HIV. Emerg Infect Dis. 2006;12(6):1025-1028. https://doi.org/10.3201/eid1206.060015. Key points:. Polymerase chain reaction. , or . PCR. , is a technique to make many copies of a specific DNA region . in vitro.  (in a test tube rather than an organism).. PCR relies on a . thermostable.