PPT-Gel Permeation Chromatography

膠體滲透層析儀 輔仁大學 化學系 化學實驗數位學習平台之建立 大綱 儀器原理 實驗圖譜分析 動畫模擬 儀器操作程序 儀器操作步驟 . PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. AP Investigation 5: Photosynthesis – Part I. Chromatography. T. echnique . used to separate a mixture into its component . molecules. The . molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Scope:. Each 400 s ITER pulse uses 120 g of T. 2. (1.2×10. 6. Ci). Release limit: 1 Ci/day; . R. elease fraction <10. -6 . for one shot. Limited Experience: 99 g processed during TFTR D-T campaign . Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Scope:. Each 400 s ITER pulse uses 120 g of T. 2. (1.2×10. 6. Ci). Release limit: 1 Ci/day; . R. elease fraction <10. -6 . for one shot. Limited Experience: 99 g processed during TFTR D-T campaign . Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . Dr. Kevin Ahern. Protein Purification. Applications of Biochemistry Knowledge. Opening Cells. Centrifugation. Fractionation. Dialysis. Applications of Biochemistry Knowledge. Separates salts from proteins. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents.