PPT-Ribozymes: RNA Enzymes - Function & Role

Pamela Osborn Overview What are ribozymes Background Structure Hairpin Hammerhead and RNase P Future applications Function amp Role of ribozymes within the body Mechanism Role of metals in catalysis amp . Definition of an enzyme. Enzyme. is . protein . . catalyst. (i.e. . increase the rate of reactions. ). . NOT. changed during the reaction. C483 Spring 2013. 1. Which . is not a difference between RNA and DNA? . A) . RNA is more prone to basic aqueous hydrolysis. . B) RNA contains uracil; DNA usually does not. . C) RNA cannot form helices. . Enzymes speed up chemical reactions.. Everything that happens in a cell. Growth. Reproduction. Interaction with the environment. Response to stimuli. Is the result of a chemical reaction.. A chemical reaction is a change in the arrangement. Stryer. Short course. Chapter 33. Nucleic Acid Structure. Nucleobase. Nucleoside. Nucleotide. Nucleic acid. Chromatin. Chromosome. Polymeric Structure. Polymer ideal for informational molecule. Ribose and deoxyribose. . ASHA S . V. BCH-10-05-05. Ribozyme. , or RNA enzyme, is a RNA molecule that act as enzymes, often found to catalyze cleavage of either its own or other RNAs.. 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. protein. !. Enzymes are . Biological . catalysts . that . increase. . the rate of metabolic reactions.. Since enzymes speed up chemical reaction rates, what are they called?. Catalyst- . A substance . Anabolism vs Catabolism. What is necessary for a chemical reaction to begin?. Reactant(s) must come together in the right orientation. With enough energy. So ho do enzymes catalyze this process?. Lowers activation energy. Enzymes. Back when we discussed proteins, we learned that one critical function of protein was to speed up chemical reactions.. Enzymes. are the proteins that increase the rate of chemical reactions. Sisters Enzyme . Video (5:47):. https://. www.youtube.com/watch?v=qgVFkRn8f10. Ricochet Science How Enzymes Work (. 1:20). https://. www.youtube.com/watch?v=UVeoXYJlBtI. AP Biology Catalase Lab Walkthrough (6:45). To the penny placed on your desk.. Observe the penny but don’t touch it. What happens?. Explain why. 2. Give the penny a . little . push. What happened?. Why did the penny move the second time and not the first time?. Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. nano. -switches to detect novel non-coding RNA expression . .  . Scott . A. . Tenenbaum.  . SUNY- College of Nanoscale Science and Engineering, Nanobioscience. The Basic sxRNA Concept. +. OR. Structurally interacting RNA . TRIzol. purification: precipitate out everything BUT the RNA. Two common methods for separation of RNA from DNA. Selective precipitation of RNA using Lithium Chloride (LiCl). Extraction with phenol buffered at an .