PPT-Gas Chromatography-Mass

Spectrometry Dr Erica Zarate Auckland Science Analytical Services Mass Spectrometry 12 June 2015 Gas chromatography Mass Spectrometry Robust More reproducible than LCMS Can be fully automated. Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Introduction. Gas chromatography is used in many research labs, industrial labs (quality control), forensic (arson and drug analysis, toxicology, etc.), environmental labs (water, soil, air), and even in the popular TV culture (crime shows like NCIS (. Evidence. 1. Class characteristic—ink analysis, paper analysis, most handwriting analysis. 2. Individual characteristic--With document analysis, it is difficult to identify to an individual characteristic except with typewriter or printer specific marks.. Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. , All right reserved 123 International Journal of Agriculture Innovations and Research Volume 6 , Issue 1 , ISSN (Online) 2319 - 1473 Gas Chromatography : Principles , Advantages and Applications i . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. .. Different substances, carried by a gas, travel through a column packed with a solid material at different speeds, so that they become separated the number of peaks on the output of a gas chromatograph shows the number of compounds . Introduction, theory, instrumentation, . derivatization. ,. Temperature programming, advantages, disadvantages and applications. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University.