The inverted scope Objectives are beneath the specimen Room to manipulate the specimen Using transmitted light Turn on the lamp Point image to eye Select A for brightfield Microscope assignment ID: 1048110
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1. Using the fluorescence microscope
2. The inverted scope
3. Objectives are beneath the specimen
4. Room to manipulate the specimen
5. Using transmitted light:Turn on the lamp
6. Point image to eye
7. Select A for brightfield
8. Microscope assignmentStage micrometer to the eye10X brightfield (A)10X phase (Ph1)40X phase (Ph2)Stage micrometer to the camera (SIDE)Adjust focusAdjust lightAdjust exposure time
9. Using emitted lightTurn on the powerIgnite the arc lampDO NOT TURN OFF
10. Viewing cellsGet cells in focus in phaseRotate filter B into placeBlue light shines on specimen, green to eyeOpen shutter (O)
11. Open this shutter, too