PPT-How Enzymes Work

Pratt amp Cornely Ch 6 Enzymes Biocatalyst Active site SubstrateProduct Reaction specificity Stereospecificity Coupled reactions Regulation Rate Enhancement Orotidine Decarboxylase. Reactions. Most reactions take place in a number of steps which need to be carefully controlled if the cell is to function properly. Enzymes are the most important controllers of cellular reactions. Catalysts. Enzymes:. They do all the work!. Enzymes . Proteins. Help . chemical . reactions happen. reduce . activation energy. increase . rate. of reaction without being . used up. R. equired. . for most biological reactions. CATALYSTS AND ENZYMES. What are catalysts?. Simply put, catalysts are substances which, when added to a reaction, increase the rate of reaction by providing an alternate reaction pathway with a lower activation energy (Ea).. IMPORTANCE. OF ENZYMES. Enzymes are biological catalysts that:. . I. ncrease. . reaction rates . by lowering the amount of energy needed for the reaction to occur. 100-1000x . faster. !!!. Activation Energy. 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. protein. !. Enzymes are . Biological . catalysts . that . increase. . the rate of metabolic reactions.. Since enzymes speed up chemical reaction rates, what are they called?. Catalyst- . A substance . Part 1. . Chapter 3. Learning outcomes. You should be able to:. explain how enzymes work. describe and explain the factors that affect. enzyme activity. use V. max. and K. m. to compare the affinity of different enzymes for their substrates. Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . Restriction enzymes. Polymerase enzyme. Strand-displacing polymerases. Helicase enzymes. Discovery and Obtainability:. Most enzymes are proteins discovered in cells.. But . DNAzymes. were . descovered. Enzymes. Back when we discussed proteins, we learned that one critical function of protein was to speed up chemical reactions.. Enzymes. are the proteins that increase the rate of chemical reactions. In order for chemical reactions to take place, . enzymes . must be present to help . speed up . the reaction.. Chemical bonds connect atoms. to make . molecules. .. Chemical . reactions. can do two things:. Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Steps in RDT:. that . catalyze. (. i.e.. , . increase or decrease the rates. of) . chemical reactions. .. . In enzymatic reactions, the . molecules. at the beginning of the process are called . substrates. , and they are converted into different molecules, called the . Carbohydrates: Bread, Pasta, Potato. Protein: Meat, Nuts, Lentils. Fats: Butter, Milk. Our body needs to digest them – turn them into a form that can be absorbed into the blood and used by cells. Enzymes make this possible..