PPT-Introduction to Proteomics

Phil Charles CCMP Overview of Talk Overview of proteomics as a concept Techniques discussion 2D Gels and experimental design paradigms Proteomics mass spectrometry Identification Quantitation. Nock Malcolm S Ball Ian R White J Mark Skehel Louisa Bill and Peter Karuso 23 GlaxoSmithKline Pharmaceuticals Gunnels Wood Road Stevenage Hertfordshire SG1 2NY UK FLUOROtechnics Pty Ltd Macquarie University Sydney NSW 2109 Australia Department o Nock Malcolm S Ball Ian R White J Mark Skehel Louisa Bill and Peter Karuso 23 GlaxoSmithKline Pharmaceuticals Gunnels Wood Road Stevenage Hertfordshire SG1 2NY UK FLUOROtechnics Pty Ltd Macquarie University Sydney NSW 2109 Australia Department o Motivation: Mass spectrometry experiments in the field of proteomics produce lists containing tens to thousands of identified proteins. With the Protein Information and Property Explorer (PIPE) the b PROTEOMICS OF ORGANELLES AND LARGE CELLULAR STRUCTURES www.nature.com/reviews/molcellbio LysosomesPeroxisomes affinity reagent Lysosome(directed towards the b CAVEOLAEA plasma-membrane invagination th Proteomics & Protein Sciences min mAU 1,000 1,500 2,000 2,500 Flow-throughLow abundant proteins Elution14 high abundant proteins International welcomes . submissions that are original and technically so as to serve both the developing world and developed countries in the best possible way.. OMICS Journals are poised in excellence by publishing high quality research. . Cress(Lepidiumsativum)/Endospermweakening/Micropylarendospermcap/Plantproteomics/Seedgermination1IntroductionSeedgerminationisde International welcomes . submissions that are original and technically so as to serve both the developing world and developed countries in the best possible way.. OMICS Journals are poised in excellence by publishing high quality research. . Kelly Ruggles, Ph.D. . Proteomics Informatics. Week 9. As the cost of high-throughput genome sequencing goes down whole genome, . exome. and RNA sequencing can be easily attained for most proteomics experiments. Fang et al., http://dx.doi.org/10.4172/jpb. 1000361 Volume 8(6) 133-141 (2015) - 133 Bcnmrs Lingling Fang 1,2 , Kyoko Kojima 3 , Lihua Zhou 2 , David K Crossman 4,5 , James A Mobley 2-4 * and Jayle Orbitrap. at the Proteomics facility of the University of Stellenbosch. Salome . Smit. Central. Analytical Facility . University. of Stellenbosch. Overview. Proteomics analysis of cardiac endothelial cells. Introduction. What is proteome?. proteome is the entire complement of proteins, including the modifications made to a particular set of proteins, produced by an organism or system at particular time and conditions.. Kate R. Rosenbloom, Hiram Clawson, Mark Diekhans, Luvina Guruvadoo, Rachel A. Harte. 1,2. , Donna Karolchik, Brian T. Lee, Brian J. Raney, Ann S. Zweig, . David Haussler. 1,3. , Robert M. Kuhn, W. James Kent. Ryan Fellers, Proteomics Center of Excellence. OSG All Hands Meeting, 3/24/2015. Agenda. Introduction to Proteomics. What is it?. Top Down Proteomics specifics. Tools . and Support. Legacy of Tool Development.