PPT-RNA Polymerase

Marvin H ONeal III PhD Wali Karzai PhD Ke Shang Class of 2012 Approach Select problem child Student outcomes in 300level BIO courses at Stony Brook Course year Total of students. nudlernyumcorg DOI 101016jcell201206003 RNA polymerase is a ratchet machine that oscillates between productive and backtracked states at numerous DNA positions Since its 64257rst description 15 years ago backtrackingthe reversible sliding of RNA poly (PCR). PCR. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone). Allows scientists to isolate pure quantities of specific DNA sequences. & 9. Polymerase Chain Reaction (PCR). General Genetics. Objectives:. 1.Introduce the students to the preparation of the . PCR. reaction.. 2.Examine the . PCR. products on . agarose. gel electrophoresis.. Kit P6 v2. January 15, 2015. DNA/Polymerase Binding Kit . P6 v2. Observed lower productive fraction with P6 compared to P5, requiring higher concentration to achieve similar productivity as P5. Goal: . & 9. Polymerase Chain Reaction (PCR). General Genetics. Objectives:. 1.Introduce the students to the preparation of the . PCR. reaction.. 2.Examine the . PCR. products on . agarose. gel electrophoresis.. Replication. Functions (Stores and provides copies of genetic material- genes). Blueprint (genes) for Protein Synthesis (Enzymes and cell building blocks). Francis Crick. RNA (Ribonucleic Acid). Why do we need RNA?. Nahla . Bakhamis. Multiple copies of specific DNA . sequences;. . ‘Molecular Photocopying’ . Polymerase Chain Reaction. 1983;. In . vitro. enzymatic amplification of specific DNA sequences from . --students should be able to:. a.) describe at the biochemical level the events that occur to go from gene to phenotype; . b.) identify different types of RNA, note their properties, how they are processed to yield a functional form, and their function in gene expression; . By: Samuel Shepler. 5’. 3’. 3. ’. 5. ’. = Thymine. Key. = Adenine. = Cytosine. = Guanine . = Phosphate. = Sugar. =DNA Helicase. 5’. 3’. 5’. 3’. To start off DNA replication, there is a 5’ end and a 3’ end. The DNA replication starts at the 3’ end and it ends at the 5’ end.. Suggested Reaction ProtocolT7 RDNA Polymerase will incorporate many di26erent 2-deoxy NTPs and 2-modi31ed NTPs except for 2-O-methyl UTP or CTP 29e e25ciency of incorporation yield and optimal reactio Traditional Enzymes Engineered VariantsThermophlic PolymerasesA Users Guide Polymerase Pedigree Polymerase Selection Guide Convenience versus FlexibilityTaq Polymerase Hot Start Polymerase Proof Biol. 1208(r). overview. Where are we today?. How does . pcr. work?. Perform . pcr. reactions. overview. Where are we today?. Initial sea-water inoculations. Back up & Grow positives to larger concentrations. PYF12 3/21/05 8:04 PM Page 191 repressor, activation of the operon by cAMP-CAP, and control of the operon bypost-transcriptional, and post-translational regulatory mechanisms and will be usedto dem A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional .