PPT-A genome-wide perspective on translation of proteins

Dec 2012 Regulatory Genomics Lecturer Prof Yitzhak Pilpel Teaching assistant Idan Frumkin idanfrumkinweizmannacil Submit Sunday at midnight The Central Dogma of Molecular Biology Expressing the genome. Denovo genome Denovo genome outline outline novogenome from contigs from assembled contigs annotation Denovo genome Denovo genome Reads contig Gene Gene Annotation Gene Annotation Forgene Jan 2012. Regulatory Genomics. Lecturer: Prof. Yitzhak Pilpel. Selection of codons might affect:. Accuracy. Throughput. Costs. Folding. RNA-structure. C. c. d. dC. cC. Fo. d*C. c*C. Free energy. l’c. BCB 660. October 20, 2011. From Carson Holt. Annotations. Automated. Ab. initio. (based on genomic sequence alone). Involves comparisons to known proteins (BLAST similarity). Sequence motifs such as start/stop . 2. Proteins (. Polypeptides. ). Chains of Amino acids (. 20. different kinds). bonded together by . peptide bonds. . (. polypeptides. ). Made of . Nitrogen, Carbon, Oxygen, and Hydrogen. Functions:. Proteins account for more than 50% of the dry mass of most cells. Protein functions include structural support, storage, transport, cellular communications, movement, and defense against foreign substances. Genome: the total number of genes in an individual.. Human Genome- approx. 20,000 genes on the 46 human chromosomes.. Human Genome Project (HGP). Ongoing effort to completely map and sequence our genome.. Nadia Khan, Rick Smith, . and Anna . Kuperman. Epigenetics 2012. Introduction. Most Genome Wide Approaches were adapted from technologies originally developed for detecting methylation at the level of a single gene. Translation. Unit 1. 1.1 Basic Definitions. To realize what the translator does in the process of translation, we must clarify a number of terms that will be used throughout the course: . Text: . Any stretch of speech or writing assumed to make a coherent whole. . Jan Pačes. Institute of Molecular Genetics AS CR. sizes of selected completed genomes. genome. chromosomes. size. genes. Mycoplasma. . genitalium. 0.58 . Mbp. 521. Escherichia coli. 4.6 . Mbp. (5.4. Most abundant molecule in cells making up 50% or more of their dry weight. Every protein has a unique structure, or shape. Allows it to perform a specific function. Enzymes are proteins that function as catalysts for many reactions in foods. Bas E. Dutilh. March 4. th. 2013. Protein function. Phenotypic. . function. E.g. . apoptosis. GO: . Biological. . process. Cellular. . function. E.g. . ribosome. GO: . Cellular. component. Molecular. T.H. Beaty. FaceBase. Meeting. June 5, 2010. Pittsburgh PA. A bit of background. Both cleft lip with/without cleft palate (CL/P) & cleft palate (CP) show strong familial aggregation. Twin & family studies show clear evidence of genetic control. G-OnRamp Beta Users Workshop. Wilson Leung. 07/2016. Outline. Obtain genome assemblies from NCBI. Transfer . large genomics datasets to Galaxy. Common bioinformatics file formats . D. atatypes in Galaxy. emm1. invasive Group A . Streptococcus. isolated from Belgian patients during 1994˗2013 . J. Coppens. 1. , . B. B. . Xavier,. . J. Sabirova. 1. , C. Lammens. 1. , K. Loens. 2. , S. Malhotra-Kumar.