PDF-Phage particle

Peptide gene fused to minor coat protein gene Phage coat proteins displaying foreign proteins Peptide gene fused to majorcoat protein gene Foreign proteins are displayed on some coat proteins Cell. Yvonne. . Lin. . AP. . Bio. . 2. a.k.a. Phages. Bacterial . VIRUS. Attacks, infects, and replicates within bacteria. Discovered. . by. . Frederick. . Twort. . (Great. . Britain, 1915). Species specific – named after the group or species it infects. LOCK. it out on the first move. HI, DROXYLATE and . SWEET RELEASE defeat METHYLATE. but stay in play until cancelled. If host plays RESTRICT. then phage can counter with. METHYLATE – move both cards to the discard pile. Gary Larson – The Far Side. ASSESSMENT. UCLA Instructional . Laboratories: Where Leading-Edge . Science Education . Is Always Bruin. Jordan Moberg Parker. Research Immersion Lab in Virology. http://www.youtube.com/watch?v=rzdwfwuVWUU. How to identify circularly permuted genomes, physical ends, 3’ overhangs, terminal repeats, and nicks.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. Also, palindromes. What are they?. Short sequences (between 4 and 13-ish nucleotides) – one might even say they’re very short. Occur multiple times in the genome, at . dispersed. intervals (not repeated right next to each other) – “distributed or spread over a wide interval”. Abdulrahman. . Alazemi. , . Shahroze. Abbas, Liam Lewis, Donald Ta, Ann Vo. Overview. Clustered repeats. Wide variety of functions. History largely unknown. Regulatory Sites. A segment capable of altering expression of specific genes. Protocol 10.1 through 10.3. Objective. : To cut phage genome into multiple fragments based on DNA sequence. General Introduction on Restriction Enzymes. Are also known as restriction endonucleases. Are naturally occurring enzymes used by bacteria for defensive purposes against extraneous DNA molecules. Yang Xin. Paper presentation. PNAS 2009, 106(12):4629-4634. Introduction. Antimicrobial drug development is increasingly lagging behind the evolution of antibiotic resistance. as a result, there is a pressing need for new antibacterial therapies that can be readily designed. . Algorithm. Decision Support. 2010-2011. Andry Pinto. Hugo Alves. Inês Domingues. Luís Rocha. Susana Cruz. Summary. Introduction to Particle Swarm Optimization (PSO). Origins. Concept . PSO Algorithm. Tail sheath. Tail fiber. DNA. 100 nm. 1. EXPERIMENT. Phage. DNA. Bacterial cell. Radioactive protein. Radioactive DNA. Batch 1: radioactive sulfur (. 35. S). Batch 2: radioactive phosphorus (. 32. P). Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. of . mycobacterial disease. Fernanda . Alonzo, . Adebanjo. . Adedoja. , . Ann . M. Findley, School of Sciences, . ULM; . Chris . R. . Gissendanner. , School of Pharmacy. , . ULM. . Abstract. Nontuberculous . or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. Dan Russell. Pauline: 7?!. Happy. Sad. 19. 2. Failure. Success. 19. 41. Star Trek. Star Wars. 9. 17. Batman. Superman. 8. 4. Mulan. Belle. 4. 4. Rapper. Scientist. 37. 43. 3. 5. 6. 10. 0. Mother. Father.