PPT-Bacterial cell Phage head

Tail sheath Tail fiber DNA 100 nm 1 EXPERIMENT Phage DNA Bacterial cell Radioactive protein Radioactive DNA Batch 1 radioactive sulfur 35 S Batch 2 radioactive phosphorus 32 P. Khansa. . Bukhari. Maryum. . Zeb. . History and Definition. Frederick . Twort. (1915) and Felix . d'Herelle. (1917) were the first to recognize bacteriophage.. In the 1930s and after words virologists utilized these viruses as model systems to investigate many aspects of virology. Yvonne. . Lin. . AP. . Bio. . 2. a.k.a. Phages. Bacterial . VIRUS. Attacks, infects, and replicates within bacteria. Discovered. . by. . Frederick. . Twort. . (Great. . Britain, 1915). Species specific – named after the group or species it infects. LOCK. it out on the first move. HI, DROXYLATE and . SWEET RELEASE defeat METHYLATE. but stay in play until cancelled. If host plays RESTRICT. then phage can counter with. METHYLATE – move both cards to the discard pile. By Monique Hunter. Bacteriophages or phages are viruses that infect bacteria.. In 1917, . French-canadian. microbiologist Felix . d’Herelle. observed an unknown substance killing bacteria as did his . . Josiah Austin, Julia Tang, Tiffany Nguyen, Michael . Ketteh. , Lynn Zhou, Dr. Mao PhD. The M13 phage is a human-friendly bacteriophage that only infects bacteria cells. 1. . It’s a nanofiber-shaped phage with five different coating proteins wrapped around its DNA (Figure 1b). The M13 phage can be engineered to display the exogenous peptides on its surface. This phage display technique allows us to study the function of short peptides that are derived from the cell’s growth factors, or proteins. Additionally, M13 phage have the ability to self-assemble into organized films that look like which can serve as a matrix for a cell culture. The phage film matrix can give us a better understanding of the role of short peptides in growth factors. The traditional method of making phage films requires the use of a pipette to drop the negatively charged phage solution onto the poly-lysine (Figure 2). However, manually, the results are not replicable and the process is time consuming. In order to make more organized and repeatable phage films we use a programmable slide stainer machine (Carl Zeiss, Inc.) that can dip the slides into a each solution allowing time to dry and giving it the ability to agitate. Resultantly, we built phage films while varying soaking time, phage concentration, and the number of phage layers to determine the best conditions to form well-ordered phage films with the help of slide stainer.. World’s Largest provider of commercial bacteriophage products for agricultural use.. Bacteriophage?. Bacteria-eater. Naturally occurring bacterial virus. Cannot infect mammalian, fungal, yeasts or molds. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. of . mycobacterial disease. Fernanda . Alonzo, . Adebanjo. . Adedoja. , . Ann . M. Findley, School of Sciences, . ULM; . Chris . R. . Gissendanner. , School of Pharmacy. , . ULM. . Abstract. Nontuberculous . In lytic cycle, the phage DNA enters the host bacterium, its genes are transcribed, the phage GM replicated, and the protein components of the phage particle are produced. . Finally, the host bacterium is lysed to release the assembled progeny particles by the process of lysis.. . DNA in mycobacterial genomes. . Bobby Chaggar. BNFO 301. Lysogeny. Group. Compare (SEQUENCE-SIMILAR-TO) each lysogenic, . mycobacteriophage. to a list of all mycobacteria in . BioBIKE. (. PhAnToMe. Rene N. Beaudoin, Danielle R. DeCesaro, Debrah L. Durkee, and Susan E. Barbaro 2 Figure 1: Basic Structure of Bacteriophage (Madigan and Martinko, 2006). nteric bacteri dreamstime.com. 3. ASFV Cameroon: what's known and key challenges. What's known . Molecular characterisation . 1. Genotype I. 2.Genotype . Ia. and . Ib. 3. . Sero. -group I. 4. Four variants . with 6,19,20 . or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. . Luis Villarreal. . M. Comar. Dip. Scienze Mediche, Chirurgiche e della Salute .