PPT-Introduction to epigenetics: chromatin modifications, DNA m

CpG Island landscape part 2 Héctor Corrada Bravo CMSC858P Spring 2012 many slides courtesy of Rafael Irizarry How do we measure DNA methylation Microarray Data One question Where do we measure . II:. Epigenetics and the histone code. Jim Noonan. GENE760. 1. TF binding. Biochemical indicators of regulatory function. 2. . Histone. modification. H3K27ac. H3K4me3. 3. Chromatin. modifiers &. Kevin Ferriter. Mariah Minder. From Yesterday…. Do you think your brain cell and your blood cell have the same DNA sequence?. Genetics. Every cell contains all of your DNA. Not every cell expresses all of your DNA. CpG. Island landscape. Héctor. Corrada Bravo. CMSC702 . Spring . 2013. (many slides courtesy of Rafael Irizarry). Genetics: the alphabet of life. Letters of DNA sequence carry the . information. How is this information read and parsed.. Joo. ’ Kim. Louisiana State University. What is . Epigenetics. ?. “the study of mitotically and/or . meiotically. heritable changes in gene function that can not be explained . by changes . in DNA sequences” – (Riggs et al. 1996). Waddington, 1942. “The study of any potentially stable, and ideally, heritable change in gene expression or cellular phenotype that occurs without changes in Watson-Crick base-pairing of . DNA” . Waddington, 1942. “The study of any potentially stable, and ideally, heritable change in gene expression or cellular phenotype that occurs without changes in Watson-Crick base-pairing of . DNA” . Epigenetics comprises (hereditary) changes in gene expression without altering the DNA sequence . Epigenetics is a biological subdiscipline that deals with questions of development, heredity, gene regulation and evolution . Nucleosome. A packaging unit for DNA (=H3/H4 tetramer + two sets of H2A/H2B . dimer. ). DNA (- charge) and . histones. (+ charge) . histones. = tails and globular domains. Higher-Order Packaging of Chromatins. Thimo Rohlf 1,4 , Lydia Steiner 1,2 , Jens Przybilla 1 , Sonja Prohaska 2 , Hans Binder 1,3 and Jörg Galle 1 * 1 Interdisciplinary Centre for Bioinformatics of Leipzig University, D-04107 Leipzig, ZOO LOGY Cell Biology Cell Regulatory Mechanisms : Chromatin Remodeling 1 Development Team Paper Coordinat or: Prof. Kuldeep K. Sharma Department of Zoology, University of Jammu Principal Investigat histones. (50%). Five major . histones. ; H2A, H2B, H3, H4 and H1 highly basic (. Arg. and Lys >25%). Roger Kornberg in 1974; . Chromatin. is made up of repeating units called . nucleosomes. , each containing 200 . BMI/CS 776 . www.biostat.wisc.edu/bmi776/. Spring 2019. Colin Dewey. colin.dewey@wisc.edu. These slides, excluding third-party material, are licensed under . CC BY-NC 4.0. by Anthony Gitter, Mark Craven, and Colin Dewey. 1. Bioinformatics III. X-ray structure of the nucleosome core particle consisting of core histones, and DNA. Top view.. www.wikipedia.org. Side view shows two windings of DNA and two histone layers. The DNA of eukaryotic organisms is packaged into chromatin, whose basic repeating unit is the . ‘Epi’: On top of. . Definition:. The . study of changes in organisms caused by modification of gene expression rather than alteration of the genetic code itself.. DNA. How many cell types are in the human body?.