PPT-Purification of the P66 Outer Membrane Protein of the Bacterium

Borrelia burgdorferi Space Grant Symposium Presentation Christopher Ramirez Debra Hansen and Petra Fromme 41721 1 Background 2 What is Lyme disease Most common tickborne illness in US . So how does one go about solving a crystal structure?. formulate . question . . . very . hard!. make . sample . cloning. , expression, . purification. make crystal . screening. , . optimisation. Ian Gluck. Mentor: Dr. Christine Kelly. OSU Dept. of Chemical, Biological and Environmental Engineering. The Biofuel Industry. The Conversion Process. Cellulose must be converted to glucose. Performed by cellulases. Course: Methods in protein chemistry. Rahman M. . Mahfuz. ur. 2012/01/11. SLU. To seperate a . particular. protin from all other proteins and cell . components. . There are many types of proteins within an ogranism . Presented by: . Seyed. . Davar. . Siadat. (. Ph.D. ). Pasteur Institute of Iran. 1. WHAT ARE OMVs. ?. BASIC RESEARC!. 1- Small spherical structures 20–250 nm in diameter. . 2- Produced by growing cells, not products of cell . (endoplasmic reticulum). Dr. Mamoun Ahram. Faculty of Medicine. Second year, Second semester, 2014-2014. Principles of Genetics and Molecular Biology. An overview. Endoplasmic reticulum (ER). It is a network of membrane-enclosed tubules and sacs (. Figure 4.13c. Figure 4.13b–c. Gram-Positive Gram-Negative . Cell Walls Cell Walls. Thick peptidoglycan. Teichoic acids. In acid-fast cells, contains mycolic acid. Thin peptidoglycan. No teichoic acids. LapA. in LPS Assembly. Aarthi Prakash. December 1, 2017. Lipopolysaccharide. Outer membrane of E. Coli. LPS . 6 Fatty Acyl Side Chains with many sugars attached. LPS bind together. Provides gel like barrier. absence of a comprehensive vaccine against this organism,important for epidemiologic monitoring and vaccinedevelopment (2). The serologic characterization scheme other were grouped into VR families. T Cell structure and Function . in Bacteria and Archaea. 1. Cell Shape and Size. 2. The . cytoplasmic. membrane and Transport. 3. Cell walls of prokaryotes. 4. Other Cellular Surface structures and inclusions. NBC2 . Biomanufacturing Steps in . Product Purification. UPSTREAM PROCESSING. . Genetically . engineered protein produced by . an organism (ex. E.coli) are grown to yield the optimal amount of product. 4._____ After one generation in N-medium, the DNA is denatured and analyzed in a d DNA forms two bands, one with heavy mechanism of DNA replication in this bacterium? a) The mechanism must be conser common cold. , which can be caused by one of the rhinoviruses, to AIDS, which is caused by HIV.. They can vary greatly in size, from approximately 5-10 kb (. Papovaviridae. , . Parvoviridae. , etc.) to greater than 100-200 kb (. Many cloning vectors have been engineered so that. the protein being expressed will be fused to another. protein, called a . tag, which can be used to facilitate. protein purification. Examples of tags include glutathione-. By: Matthew Copher, Corey Schmidt, & Hunter . Tessman. Abstract:. Background:. In this study the goal was to extract and purify lysozyme from the shell of a hen egg along with the membrane and determine the enzymatic activity and efficiency of lysozyme..