PPT-Purification of the P66 Outer Membrane Protein of the Bacterium

Borrelia burgdorferi Space Grant Symposium Presentation Christopher Ramirez Debra Hansen and Petra Fromme 41721 1 Background 2 What is Lyme disease Most common tickborne illness in US . So how does one go about solving a crystal structure?. formulate . question . . . very . hard!. make . sample . cloning. , expression, . purification. make crystal . screening. , . optimisation. Ian Gluck. Mentor: Dr. Christine Kelly. OSU Dept. of Chemical, Biological and Environmental Engineering. The Biofuel Industry. The Conversion Process. Cellulose must be converted to glucose. Performed by cellulases. Course: Methods in protein chemistry. Rahman M. . Mahfuz. ur. 2012/01/11. SLU. To seperate a . particular. protin from all other proteins and cell . components. . There are many types of proteins within an ogranism . HIC Chromatography. Chromatography. A technique used to separate molecules based on how they tend to cling to or dissolve in various solids, liquids, and gases.. Characteristics of Proteins. Hydrophilic. Presented by: . Seyed. . Davar. . Siadat. (. Ph.D. ). Pasteur Institute of Iran. 1. WHAT ARE OMVs. ?. BASIC RESEARC!. 1- Small spherical structures 20–250 nm in diameter. . 2- Produced by growing cells, not products of cell . Genetic engineering. involves manipulating genes for practical purposes . Gene cloning. leads to the production of multiple identical copies of a gene-carrying piece of DNA. Recombinant DNA. is formed by joining DNA sequences from two different sources. (endoplasmic reticulum). Dr. Mamoun Ahram. Faculty of Medicine. Second year, Second semester, 2014-2014. Principles of Genetics and Molecular Biology. An overview. Endoplasmic reticulum (ER). It is a network of membrane-enclosed tubules and sacs (. Interactions. June 6, 2017. Why PPI?. Protein-protein interactions determine outcome of most cellular processes. Proteins which are close homologues often interact in the same way. Protein-protein interactions place evolutionary constraints on protein sequence and structural divergence. Iman . Alshehri. Introduction . Iman . Alshehri. In this course, you will use lactate dehydrogenase (LDH) as the subject of . your studies. . LDH (. E.C. . 1.1.1.27) catalyzes the nicotinamide . cofactor-dependent. Alex Xenopoulos*. Alison Dupont, Christopher . Gillespie. , Ajish Potty, Michael Phillips. Processing Technologies. Merck Millipore. Bedford, MA (USA). Integrated Continuous Biomanufacturing. A new ECI conference. June 12, 2018. Why PPI?. Protein-protein interactions determine outcome of most cellular processes. Proteins which are close homologues often interact in the same way. Protein-protein interactions place evolutionary constraints on protein sequence and structural divergence. LapA. in LPS Assembly. Aarthi Prakash. December 1, 2017. Lipopolysaccharide. Outer membrane of E. Coli. LPS . 6 Fatty Acyl Side Chains with many sugars attached. LPS bind together. Provides gel like barrier. Many cloning vectors have been engineered so that. the protein being expressed will be fused to another. protein, called a . tag, which can be used to facilitate. protein purification. Examples of tags include glutathione-. By: Matthew Copher, Corey Schmidt, & Hunter . Tessman. Abstract:. Background:. In this study the goal was to extract and purify lysozyme from the shell of a hen egg along with the membrane and determine the enzymatic activity and efficiency of lysozyme..