and Phagocytosis Undergraduate Graduate Histology Lecture Series Larry Johnson Professor Veterinary Integrative Biosciences Texas AampM University College Station TX 77843 VIBS 443 and VIBS 602 ID: 1011572
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1. 5.Transport, Endocytosis, and PhagocytosisUndergraduate – Graduate Histology Lecture SeriesLarry Johnson, ProfessorVeterinary Integrative BiosciencesTexas A&M UniversityCollege Station, TX 77843VIBS 443 and VIBS 602
2. Cell membraneCell membrane – plasmalemma – 8.5-10 nm
3. Cell membrane Necessary for life Marks limits of cell (defines cell) Separates cell from its environment Can not be a complete barrier, some items must pass.
4. Cell membrane Four classes of items that must pass - Nutrients (AA, O2, fatty acids, CHO, etc.,) - Waste (CO2 and products of metabolism) - Informational molecules (hormones, growth and chemotaxic factors) - Cell products (secretions, bacteria, RBC {250 billion cells/day})
5. Cell membranePlasmalemma - 8.5 - 10 nm FunctionContain antigenic molecules, which are the basis of cell recognition and tissue specificityRegular internal environment - ion pumps
6. Cell membranePlasmalemma - 8.5 - 10 nm FunctionPossess receptors for hormonesPossess mechanisms for generating messenger molecules that activate the cell’s physiological responses to stimuli
7. HISTO 61
8. Transport of small moleculesacrossmembranes Simple diffusion (cross freely) Passive, facilitated, and Active transport
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11. Simple diffusion (cross freely) Passive, facilitated, and Active transport
12. Specific (high affinity) but can be saturated
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19. http://www.youtube.com/watch?v=JShwXBWGMyY&feature=related
20. Exocytosis - release of secretory products into extracellular compartmentMembrane-bound granules move to surfaceGranule membranes fuse with plasma membraneContents flow extracellularlyInvaginating vesicles - remove excess membrane from cell membraneMinute vesicles like pinocytosis vesicles
21. ExocytosisEndocytosisBuddingCellmembrane
22. MerocrineApocrine
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24. http://www.youtube.com/watch?v=K7yku3sa4Y8&feature=related
25. Transport of macromolecules: endocytosisFluid (or bulk) flowsReceptor mediated endocytosisSpecific receptorCoates pits (clathrin)Lysosomal fusion with endosomes
26. BULK FLOW
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32. LDL and receptor separates at lower pH
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36. Insulin receptors do not recycle!clathrin coated vesicle (dots label clathrin coat) uncoated vesicle (no dots)
37. Endocytosis - bulk uptake of materials Pinocytosis - cell drinking Phagocytosis - cell eatingAttachment – binding of particle ligand to membrane receptorsInteriorization by pseudopods
38. Ingestion of particles: phagocytosis Mononuclear phagocyte system macrophageOpsonized particlesMembrane zippering mechanism
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42. Primary lysosomeSecondary
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49. SER
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51. U.S.A.Mexico
52. Cytoskeleton and cell motility Next time
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55. Bruce Alberts, et al. 1983. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY.Bruce Alberts, et al. 1994. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY.William J. Banks, 1981. Applied Veterinary Histology. Williams and Wilkins, Los Angeles, CA.Hans Elias, et al. 1978. Histology and Human Microanatomy. John Wiley and Sons, New York, NY.Don W. Fawcett. 1986. Bloom and Fawcett. A textbook of histology. W. B. Saunders Company, Philadelphia, PA.Don W. Fawcett. 1994. Bloom and Fawcett. A textbook of histology. Chapman and Hall, New York, NY.Arthur W. Ham and David H. Cormack. 1979. Histology. J. S. Lippincott Company, Philadelphia, PA.Luis C. Junqueira, et al. 1983. Basic Histology. Lange Medical Publications, Los Altos, CA.L. Carlos Junqueira, et al. 1995. Basic Histology. Appleton and Lange, Norwalk, CT.L.L. Langley, et al. 1974. Dynamic Anatomy and Physiology. McGraw-Hill Book Company, New York, NY.W.W. Tuttle and Byron A. Schottelius. 1969. Textbook of Physiology. The C. V. Mosby Company, St. Louis, MO.Leon Weiss. 1977. Histology Cell and Tissue Biology. Elsevier Biomedical, New York, NY.Leon Weiss and Roy O. Greep. 1977. Histology. McGraw-Hill Book Company, New York, NY.Nature (http://www.nature.com), Vol. 414:88,2001.A.L. Mescher 2013 Junqueira’s Basis Histology text and atlas, 13th ed. McGrawDouglas P. Dohrman and TAMHSC Faculty 2012 Structure and Function of Human Organ Systems, Histology Laboratory Manual - Slide selections were largely based on this manual for first year medical students at TAMHSCMany illustrations in these VIBS Histology YouTube videos were modified from the following books and sources: Many thanks to original sources!