PPT-Lecture 2 Recombinant DNA

Technology and Biotechnology FE314 Biotechnology Spring 201 7 Technique of manipulating the genome of a cell or organism so as to change the phenotype desirably What is recombinant DNA . Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Objective. Students will model the process of using restriction enzymes and plasmids to form recombinant DNA.. Background Information. major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes.. Will Herrick. Peyton Group Meeting. March 20, 2013. What Are Adenoviruses?. Linear . dsDNA. virus. 80-100 nm. Nonenveloped. Icosahedral structure:. 1: penton . capsomeres. 2: . hexon. . capsomeres. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Synthetic . Nucleic Acid Molecules . (NIH Guidelines). Northern Arizona University. Office of Regulatory Compliance. Shelley Jones, Director of Biological Safety. Shelley.Jones@nau.edu. 928-523-7268. Definition. Steps . Applications. INTRODUCTION. Recombinant DNA.  (. rDNA. ): .  . DNA.  molecules formed by laboratory methods of . genetic recombination.  (such as . molecular cloning. ) to bring together genetic material from multiple sources, creating . BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . Genetic engineering involves inserting foreign genes or modifying the activity of existing genes. . This technology exploits the process of gene cloning which involves making many identical copies of a gene. . TECHNOLOGY 1 Objectives • Recombinant DNA • Probes • Restriction map • Gene cloning • Gene library • Cloning vectors • RFLP • DNA Fingerprinting • DNA foot printing • Genomic impri great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . Technology. . and. . Biotechnology. II. . FE314. -. . Biotechnology. Spring . 201. 6. Some. . applications. of . recombiant. DNA . technology. in . Biotechnology. *. Diabates-Insulin. . production. Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms. Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.