PPT-Focusing the microscope simple staining

How to Focus the Microscope Turn the revolving nosepiece to 10 magnifying lens Move the stage up the condenser to the top position and close iris diaphragm half way Put the slide on the slide holder on the stage. Microscopes are delicate and expensive instruments they should be handled with utmost care Before you use the microscope your instructor will explain its proper use Following are rule s that will protect the microscope and insure that you can make m This model comes with color corrected infinity optical system (CSIS), a movable large working distance condenser, 45 degree inclined trinocular head, high eye-point Plan eyepieces, infinity long working distance PLAN achromatic objectives and infinity long working distance PLAN phase contrast objectives. This microscope offers nine brightfield magnification powers, 40X, 60X, 100X, 150X, 200X, 300X, 400X, 600X & 900X, and six phase contrast settings, 100X, 150X, 200X, 300X, 400X & 600X. Its 30 watt adopt Kohler illumination system was designed for 90-240V wide voltage power supplies. HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . CONCEPTS EXPLORED IN THIS LESSON. ocular lens / eyepiece. body tube. arm. base. light source/illuminato. r. revolving/rotating nosepiece. objective lenses. coarse adjustment knob. fine adjustment knob. From its humble beginnings the microscope has undergone numerous revisions since Leeuwenhoek’s day. But its usefulness has certainly not diminished. Numerous types of Microscopes are currently being used. Visit the following for a more detailed description of them. . Kumud Singh,. Affiliation: . Bhabha. Atomic Research Centre. PIP-II Technical Meeting. 19 Jan 2016. Outline. K.Singh. - SSR2 Focusing Lens Design. 2. 01/19/2016. Introduction. EM design and parameters. Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. How to Focus on Specimen. Turn on light source on high. Turn the objective to low (4x). Lower the objective lens so that it is close but not touching the slide. Center the image by moving the stage. Adjust the diaphragm for optimum light. Light Microscope. Simple – uses a single lens. Compound – uses a set of lenses or lens systems. Simple Light Microscope. Compound Microscope. Mechanical Parts. Used to support and adjust the parts. Light Microscope. Simple – uses a single lens. . Compound – uses a set of lenses or lens systems (this is the type of microscope we use in class, it has the eyepiece lens (ocular) and the objective lens). A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . XP-100 XP100 is extremely useful for Iatrologyand and industry fields. This microscope is good at identifying crystals suspended in liquid, identifying minerals in core samples, detecting defects in Amina Mustafa. Department of Molecular Biology. Virtual University of Pakistan. Invented by :. Antonie. van Leeuwenhoek. (Father of Microbiology). Microscopy. USES:. Diagnostics. Identification. Histology – Tissue analysis. This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such.