PPT-Figure Figure. Genetic relationships of Candida auris isolates based on whole-genome sequencing

Hamprecht A Barber AE Mellinghoff SC Thelen P Walther G Yu Y et al Candida auris in Germany and Previous Exposure to Foreign Healthcare Emerg Infect Dis 201925917631765 httpsdoiorg103201eid2509190262. Venter et. al (2004). Presented by. Ken . Vittayarukskul. Steven S. White.. Context of the Problem . Evolutionary history is directly tied to microbial genetics. Little is known. Until recently, microbial diversity was measured by PCR amplification and sequencing of only ribosomal genes. Infection Prevention and Control for . Home Healthcare and Hospice. New York State Department of Health . July . 2017. Outline. Background. Candida . auris. NYSDOH activities to help control . Candida . (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. Infection Prevention and Control for . Healthcare Providers in Dialysis Settings. New York State Department of Health . July . 2017. Outline. Background. Candida . auris. NYSDOH activities to help control . Emily Lutterloh, MD, MPH. Director, Bureau of Healthcare Associated Infections. Division of Epidemiology. New . York State Department of Health. Outline. Background and emergence. Reason for concern. Infection Prevention and Control for . Emergency Medical Services. New York State Department of Health . July . 2017. Outline. Background. Candida . auris. NYSDOH activities to help control . Candida . DJ Shannon, MPH, CIC. Infection . Preventionist. Eskenazi. Health. Objectives. 2. Understand the importance of . Candida . auris. . detection and surveillance. Identify global and US dissemination of . Whole genome sequencing of babies REASONS FOR USING WHOLE GENOME SEQUENCING IN BABIES There are a number of possible reasons for carrying out whole genome or exome sequencing Seeking a diagnosis for a Ben-Ami R, Berman J, Novikov A, Bash E, Shachor-Meyouhas Y, Zakin S, et al. Multidrug-Resistant Candida haemulonii and C. auris, Tel Aviv, Israel. Emerg Infect Dis. 2017;23(2):195-203. https://doi.org/10.3201/eid2302.161486. Katherine Buechel BSN, RN, CIC │ April 14, 2022. First Case of . C. auris . identified in TN . The TN Dept of Health announced the first case of . Candida auris . on January 27, 2022 identified at the Tennessee State Public Health Laboratory . Figure 8.01. Sequencing—Fragments of All Possible Lengths. During chain termination or . Sanger sequencing. , the target DNA fragments are copied millions of times, but each copy ends at a different nucleotide position. These subsets of fragments end with a fluorescently-labeled nucleotide that reveal the identity of the final base. The final sequencing data are a series of fluorescent peaks that correspond to the original template . Álvarez-Narváez S, Giguère S, Cohen N, Slovis N, Vázquez-Boland JA. Spread of Multidrug-Resistant Rhodococcus equi, United States. Emerg Infect Dis. 2021;27(2):529-537. https://doi.org/10.3201/eid2702.203030. Figure 9.01. PCR Detection of . Sequence Tagged Site. Mapping large genomes requires many . genetic markers. . Those based solely on their unique sequences are known as sequence tagged sites (STS). These unique sequences may be amplified by PCR and mapped relative to each other. In practice, each STS is defined by a pair of specific PCR primers at its ends that generate a PCR fragment of defined length.. Salzberg SL, Kingsford C, Cattoli G, Spiro DJ, Janies DA, Aly MM, et al. Genome Analysis Linking Recent European and African Influenza (H5N1) Viruses. Emerg Infect Dis. 2007;13(5):713. https://doi.org/10.3201/eid1305.070013.