PPT-Genome generation

Timetable for today Time Activity 0000 Introduction to the session 0010 Story cards 0020 Info cards 0030 Issue cards 0040 Discuss two key issues 0045 Feedback your issues What is DNA. AgBiotech. 4. th. International Conference on Agriculture and Horticulture. Beijing, July 13, 2015. Andy Renz, Vice President Business Development. Overview. Scope. 1. st. Generation ag biotech – . Sequencing strategies. Hierarchical genome shotgun HGS – Human Genome Project. “map first, sequence second. ”. clone-by-clone … cloning is performed twice (BAC, plasmid). Sequencing strategies. What has worked and what may work for AHC. Erin L. Heinzen, Pharm.D, Ph.D. Center for Human Genome Variation. Duke University School of Medicine. July 22, 2011. e.heinzen@duke.edu. EPILEPSY DISORDERS. Assembly, and Alignment Methods . Andy Nagar. Agenda. Background. Next Generation Sequencing. Sequence Assembly. Sequence Alignment. Traditional Alignment Algorithms. Next Generation Alignment Algorithms. BIOST 2055. 04/06/2015. Last Lecture . Genome-wide association study has identified thousands of disease-associated loci. Large consortium performs meta-analysis to further increase the sample size (power) to detect additional loci. Elaine R. . Mardis. – 11 . February. . 2008. Washington School of Medicine, Genome Sequencing Center.. Presented by . Jacob . Juhn. “If one accepts that the fundamental pursuit of genetics is to determine the genotypes that explain phenotypes, the meteoric increase of DNA sequence information applied toward that pursuit has nowhere to go but up.”. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Genome: the total number of genes in an individual.. Human Genome- approx. 20,000 genes on the 46 human chromosomes.. Human Genome Project (HGP). Ongoing effort to completely map and sequence our genome.. Hierarchical genome shotgun HGS – Human Genome Project. “map first, sequence second. ”. clone-by-clone … cloning is performed twice (BAC, plasmid). Sequencing strategies. Whole genome shotgun WGS – Celera. Cynthia N Perry, PhD. Assistant Academic Dean of Admissions. Assistant Professor, Molecular Medicine. Department of Medical Education. Foster School of Medicine. https://. youtu.be. /KiQgrK3tge8. Genomics and Healthcare. Christopher Henry, Matt DeJongh, Aaron Best, Ross Overbeek, and Rick Stevens. Presented by: Christopher Henry. Pathway Tools Workshop. October, 2010. Metabolic Modeling is One Key to Predicting Phenotype from Genotype. G-OnRamp Beta Users Workshop. Wilson Leung. 07/2016. Outline. Obtain genome assemblies from NCBI. Transfer . large genomics datasets to Galaxy. Common bioinformatics file formats . D. atatypes in Galaxy. Nathan Clement. Computational Sciences Laboratory. Brigham Young University. Provo, Utah, USA. Next-Generation Sequencing. Problem Statement . Map next-generation sequence reads with variable nucleotide confidence to .