and Molecular Genetics Toxic elements Cadmium Mechanisms of tolerance and accumulation Passage of metals from soil to plant Distribution Translocation Absorption Enhanced processes In hyperaccumulators ID: 797667
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Slide1
Laboratory
of Plant
Physiology
and
Molecular
Genetics
Slide2Toxic elements - Cadmium
Mechanisms of tolerance and accumulation
Passage of metals from soil to plant
Distribution
Translocation
Absorption
Enhanced processes
In hyperaccumulators
translocation of TME to shoots
uptake systems of TME
detoxification of TME
unloading of TME
Study of metal hyperaccumulation
reveals key nodes of the metal homeostasis network that, when altered, lead to drastic changes in metal accumulation and tolerance
TME
: Trace
metallic
element
Our aim is to decipher Cd accumulation and detoxification pathways
Slide3South-east zone:
I30 (NM) - Italy
I16 (M) - Italy
Hybrid zone:
PL34 (
NM) -
Poland
PL22 (M) - Poland
Populations studied
Arabidopsis
halleri
Slide4Genes, Metabolites and Network analyses
Network analysis: Genes that showed similar expression
patterm
Growth test on knockout mutants
(they DO NOT express a gene of interest)
WT
Mutant
Cd treatment
WT
Mutant
Oxidative stress
Slide55
Plant Circadian Clock
= ~24 hours
Slide66
Gene
expression analysis by Luminescence
Slide77
Mg and circadian oscillations
***
***
**
ns
ns
ns
***
Luminescence of plant
CCA1
promoter
*
p
< 0.05**
p < 0.01*** p < 0.001
= ~24 hours
= ~30 hoursMelo etal 2016 unpublished
Slide8Masters thesis: Analysis of clock components involved in Mg deficiency response
Role of
circadian
clock inplant adaptation to mineral stress
?
Haydon
etal
2013 Nature
502:689–692