PPT-  Staining of Fungus by using

  Staining of Fungus by using Lactophenol cotton blue Aim To stain fungal hyphae by using Lactophenol cotton blue Requirement Young fungal culture Glass slide Cover slip Needle Lactophenol. SHEET 330 - FUNGUS GNATS poorflyersgoodflyersdarkheadcapsuledarkheadcapsuleLifeCyclecompletelifecycletakesaboutweeksF).Thereareusuallycontinuousoverlappinggenerationsingreenhouses.Femaleslayeggsnearth Neg a INTRODUCTION Negative staining is an easy, rapid, qualitative method for examining the structure of isolated organelles, individual macromolecules and viruses at the EM level. However, the meth HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu Here is a nice big chunk of Tinder Funguslive birch tree. This picture shows the Tinder Funguscut open to reveal the red-brown interior Here's a small Tinder Fungus growing farther up a live birch t Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. Chapter 19. Characteristics. Fungi are . eukaryotic heterotrophs. Along with bacteria they are major . decomposers. Many are . saprophytes. which means they live off . dead. material.. Most reproduce both . Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . It has been made with all-natural ingredients that are clinically tested and proven to be effective against fungal infections. Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme Oates, N.C., . Abood. , A., . Schirmacher. , A.M., Alessi, A.M., Bird, S.M., Bennett, J.P., Leadbeater, D.R., Li, Y., . Dowle. , A.A., Liu, S., . Tymokhin. , V.I., Ralph, J., McQueen-Mason, S.J., Bruce, N.C., “. This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.