PPT-Recombinant DNA

Bacterial Transformation Making Competent Cells 1 Competent cells Competent cells are bacterial cells that possess more easily altered cell walls by which foreign DNA can be passed through easily Most types of cells cannot take up DNA efficiently unless they have been exposed to special chemical or electrical treatments to make them competent . Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Brief Chapter Outline.  . I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy. Cutting . and Joining DNA. DNA . Cloning. V. Cloning Vectors. A. . Bacterial Vectors. Synthetic . Nucleic Acid Molecules . (NIH Guidelines). Northern Arizona University. Office of Regulatory Compliance. Shelley Jones, Director of Biological Safety. Shelley.Jones@nau.edu. 928-523-7268. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. . Hameed. Abdullah. (. or . rDNA. ) is made by combining DNA from two or more sources. In practice, the process often involves combining the DNA of different organisms. . The . process depends on the ability of cut, and re-join, DNA molecules at points identified by specific sequences of nucleotide bases called restriction sites. DNA fragments are cut out of their normal position in the chromosome using . A deadly pathogen called the papaya . ringspot. virus (PRV) had spread throughout the islands. . It appeared poised to completely decimate the papaya plant population.. Scientists from the University of Hawaii were able to rescue the industry by creating new, genetically engineered PRV-resistant strains of papaya.. BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.. Concept and basic steps in recombinant DNA technology and gene cloning. -2-. Recombinant DNA technology is playing a vital role in improving health conditions by . developing new vaccines and pharmaceuticals. Technology AQC - 321 Dr. Mamta Singh Assistant Professor COF (BASU), Kishanganj Recombinant DNA Technology... Definition : It is a technology of joining together of DNA molecules from two different s Technology. . and. . Biotechnology. II. . FE314. -. . Biotechnology. Spring . 201. 6. Some. . applications. of . recombiant. DNA . technology. in . Biotechnology. *. Diabates-Insulin. . production. Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.