PPT-Control of Eukaryotic Gene Transcription

Teachable Unit Gene Expression Team NANSI 2013 University of Minnesota Participants Michael Burns Lucy He David Kirkpatrick Bridget Lear Tamar Resnick Turk Rhen Facilitators Judy Ridgway Sue Wick. 0. Chapter 15. Eukaryotic Gene Regulation. Almost all the cells in an organism are genetically identical.. Differences between cell types result from . differential gene expression. , the expression of different genes by cells with the same genome.. for Biotechnology. Central Dogma. The direction of the . flow of genetic information. is from DNA to RNA to polypeptide.. DNA Replication. Initiation and Unwinding of DNA. DNA . gyrase. . (. topoisomerase. Topic 2.6 & 7.2. Understandings:. Transcription is the synthesis of mRNA copied from the DNA base sequences by RNA polymerase. . Gene expression is regulated by proteins that bind to specific base sequences in DNA.. Chapter 13. Prokaryotic . REgulation. 13.1 . Prokaryotic Regulation. Bacterium don. ’. t always need to express all enzymes and proteins. . Think about the cool lights in the cooler section at a grocery store. They only turn on when you open the door or walk by. . Eukaryotic Cells – cells with a nucleus and many organelles. Organisms with nucleated cells belong to the largest classification group, Domain . Eukaryota. Four Kingdoms of Organisms which are eukaryotic are animal, plants, . Anablep. . anablep. . with its “4 eyes”. Upper half of lids look aerially while the lower half looks into the water.. Cells of the two parts of the eye exhibit differential gene expression. Individual bacteria respond to environmental change by regulating their gene expression. TUMOR SUPRESSOR PROTEINS. ATP. ENZYMES THAT CONTROL CELLULAR RESPIRATION. ADAPTIVE GENE PRODUCTS ARE ONLY REQUIRED UNDER CERTAIN CONDITIONS. GENES FOR PROTEIN DIGESTION ARE TURNED OFF WHEN THERE IS NO PROTEIN AVAILABLE. . 10.1 Between You and Eternity. Cancer is a multistep process in which cells grow and divide abnormally, disrupting physical and metabolic functions. More . than 200,000 new cases of breast cancer are diagnosed in the US each year – about 5,700 in women and men . Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. . 1. The . trp. Operon. Contains 5 genes coding for proteins (enzymes) required for the synthesis of the amino acid tryptophan. Also contains a promoter and operator.. When tryptophan levels in the cell are low, the expression of the . Central Dogma. The direction of the . flow of genetic information. is from DNA to RNA to polypeptide.. DNA Replication. Initiation and Unwinding of DNA. DNA . gyrase. . (. topoisomerase. ) unwinds the DNA coil.. 5 mechanisms . Chromatin Structure. Transcriptional. Posttranscriptional. Translational. Posttranslational. Chromatin Structure. Which of these is more accessible to the kitten?. A. Or. B. Figure 15.7. . RNA. . transcription . &. Post transcription events. General differences between transcription and replication. Replication. Transcription. purpose. The purpose of replication is to conserve the entire genome for next generation.. Acknowledgement. Centers for Disease Control and Prevention- Ethiopia (CDC-E). American Society for Clinical Pathology (ASCP). Addiss. Ababa University . University of Gondar. University of . Hawassa.