PPT-Hemispherical Confocal Imaging using Turtleback Reflector

Yasuhiro Mukaigawa Osaka University Seiichi Tagawa Osaka University Jaewon Kim MIT Media Lab Ramesh Raskar MIT Media Lab Yasuyuki Matsushita Microsoft Research Asia Yasushi Yagi Osaka University. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Cell-Nucleus_stack.avi. Confocal Laser Scanning . Microscpy. image stack of a . Chironomus. . tentans. salivary gland cell nucleus. The fluorescence was due to the protein hrp36 conjugated to red-fluorescent AlexaFluor647, which was microinjected into the cytoplasm. After transport into the nucleus, hpr36 was incorporated into nascent RNA-particles, such that active transcription sites were marked. The brighter oval region in the image center is the cell nucleus. The . BIF Microscopy Workshop. March 25. th. , 2015. Director: Professor Thomas V. O’Halloran. Managing Director: Keith MacRenaris, . Ph.D. History and Mission of CLP and QBIC. “The . Chemistry of Life Processes Institute acts as an umbrella for a variety of centers, facilitates collaborations and helps bridge different cultures. By lowering the barriers to scientific discovery, the Institute hopes, for example, to design new drugs for the treatment of cancer and neurodegenerative diseases as well as develop improved techniques for diagnosing diseases earlier.” . Ankit. Jain. B.Tech. 5. th. . Sem. (ECE). IIIT Allahabad. Features :. High Gain ( 30-40 db). Low cross polarization. Reasonable bandwidth, Fractional Bandwidth being at least 5% on commercial models.. Confocal Application Notes Vol. 5 May 2007 Leica Microsystems Inc. Toll Free 866-830-0735, Option 3 Application and Technical Support Fax (610)-321-0425 410 Eagleview Blvd, Ste. 107 www.conf Confocal Application Notes Vol. 4 October 2006 Leica Microsystems Inc. Telephone (610)-321-0460 410 Eagleview Blvd, Ste. 107 Fax (610)-321-0425 www.confocal-microscopy.com Exton, PA 19341 2 lecture. 16. November 2011. Fourier. plane. Point . object. Image. f. f. f. f. a. a. ’. Magnification. : m=1. Angles. : sin(. a. ’)=sin(. a) .  . a. ’=. a. Magnification and resolution: the Abbe limit. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. http://www.glass-bottom-dishes.com/ Medium aging where fluorescent molecules are Information regarding protein mobilitymeasuring the rate of fluorescenc Why Does Fluorescence Recover? Pre-bleachPartia Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . :-;.*89! ! ! !"##$% & '&()%*#$+%#, & '&(-*."%/.#, & 0%*/ & 12 & 0%3"*/ & 0"/ & 0*4 & 5-".6%7"$) & 89 & : & 8;9 & =;�8 & 8;=? & @;A9 & AB;8B & +)*7 & B= & ? & A;@B & @;�A & @;B & @;@= & : 2 4 Microscope objectives are always labeled with their magnification and numerical aperture (N.A.). The numerical aperture is defined by where is the refractive index for the medium betwee JSC Innovation Charge Account Project. Toni A. Clark, P.E.. Max Martell. JSC Lighting Lab. ICA Project Overview. This project investigated passive (non-powered) lighting countermeasures to improve lighting conditions where the Sun’s rays are nearly horizontal with the operational surface..