PPT-Introduction to RNA-

Seq and Transcriptome Analysis Hands on activities Fun with UNIX PowerPoint Jessica Kirkpatrick and Casey Hanson RNA Seq Lab Jessica Kirkpatrick 2015 1 Exercise Use the . USER GUIDE For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Information in this document is subject to change without notice.DISCLAIMERSLIFE TECHNOLOGIES CORPO 7. 7 RNA Synthesis and Processing. Chapter Outline. Transcription in Prokaryotes. Eukaryotic RNA Polymerases and General Transcription Factors. Regulation of Transcription in Eukaryotes. RNA Processing and Turnover. DNA. RNA. PROTEIN. RNA and Transcription. RNA. R. ibo. n. ucleic . A. cid. another type of nucleic acid similar to DNA found in cells. also a polymer made of nucleotides. takes information from the nucleus to the rest of the cell. DNA. Replication. Transcription. Translation. Polymerase. Monomers. DNA Pol III . (and I). dNTPs. Direction . of . synthesis. 5. ’. to . 3’. Template. ssDNA. Product. polynucleotide. RNA. Protein. Gene . to . Phenotype. : . The BAD2 gene. . and . fragrance in rice. DNA . sequence specifying . a . protein 200 . – 2,000,000 . nt. (. bp. ). Eukaryotic Gene Structure. Ribonucleic acid (RNA) is a key nucleic acid in transcription and translation. RNA is like DNA except that:. SUBMITTED BY. . SELMA ABDUL SAMAD. . BCH-10-05-02. . S3 . MSc. BIOCHEMISTRY. Interrupted Genes. Prokaryotes. – continuous gene(uninterrupted) . Eukaryotes. – gene is interrupted with non-coding sequences (. Chapters 16 and 17. Before the end of the semester we will be covering…. Historical DNA experiments. Structure of DNA/RNA. DNA Replication. Protein Synthesis (Transcription and Translation). Mutations. Add to Table . of Contents. p. 10 DNA/RNA Vocabulary. p. 11 DNA/RNA Structures. p. 12 Complementary Base-Pairing. DNA. Deoxyribonucleic Acid. Double-stranded. Double-helix structure. Made of nucleotides - each nucleotide has 3 parts (remember PBS). Chapter 13 . (Pgs 360-389 Miller and Levine Biology text. ). http://www.youtube.com/watch?v=suN-sV0cT6c. . Where have we seen DNA being replicated?. MITOSIS AND . MEIOSIS. Proteins are molecules that are necessary for virtually every activity in your body. Some of the Biological Functions of Proteins include:. CHM . 341. Suroviec. . Fall . 2016. I. . Nucleotides, Nucleic Acids and Bases. Bases. Planar, aromatic, heterocyclic. Purine (2 rings). Pyrimidine (1 ring) . Adenine (A). Guanine (G). Thyamine (T). Rescues C9-ALS/FTD Molecular Hallmarks . In Vivo. and . In Vitro. Katharina E. Meijboom. 1. , Nicholas Fordham. 1. , Meghan Blackwood. 1. , Gabriela Toro Cabrera. 1. , Robert H. Brown. 2. , Christian Mueller. Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. .. The length of the transcription bubble is about 12 to 14 . bp. , but the length of the RNA–DNA hybrid within the bubble is only 8 to 9 . bp. . As the enzyme moves along the template, the DNA duplex reforms. TRIzol. purification: precipitate out everything BUT the RNA. Two common methods for separation of RNA from DNA. Selective precipitation of RNA using Lithium Chloride (LiCl). Extraction with phenol buffered at an .