AMOEBA Protozoa The protozoa are a diverse group of unicellular eukaryotic organisms Many have evolved structural features organelles that mimic the organs of multicellular organisms Reproduction is generally by mitotic binary fission although in some ID: 497151
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Slide1
PROTOZOA
AMOEBASlide2
Protozoa:
The protozoa are a diverse group of unicellular, eukaryotic organisms. Many have evolved structural features (
organelles
) that mimic the organs of
multicellular
organisms. Reproduction is generally by mitotic binary fission, although in some
protozoal
species, sexual (meiotic) reproduction with several variations occurs as well. Only a few of the many tens of thousands of protozoan species are pathogenic for humans.Slide3
Clinically important protozoa:
Many of these protozoa have both a dormant, immotile
cyst
stage (the infective stage) that permits survival when environmental conditions are hostile, and a motile, actively feeding and reproducing vegetative (
trophozoite
) stage (diagnostic stage). For convenience, the protozoa are classified according to their mode of locomotion. The clinically relevant protozoa are thus divided into four groups.Slide4
Classification of protozoa:
Either by mode of
locomotion:Slide5
Or by site where
infection occurs : Slide6
AMOEBA:
Can be either free living or parasitic.
Parasitic Amoeba: the parasitic is either pathogenic:
Entamoeba
histolytica
, or non-pathogenic:
Entamoeba
coli,
Iodamoeba
butschlii
,
Endolimax
nana.
Pathogenic free living amoeba
:
Naegleria
and
Acanthamoeba
sp.Slide7
Entamoeba
histolytica
:
We can see 2 stages : Cyst,
trophozoite
.
Cyst: define cell wall, small, from 1-4 nuclei, the
karyosome
is in the center of the nucleus.
Trophozoite
: start to motion, change its cell wall to elongated, have pseudopodia, still small, with 1 nuclei contain also
karyosome
in the center.Slide8
Glass slides for E.histolytica
Trophozoiate
Trophozoite
Slide9
Entamoeba
histolytica
Cyst
Cyst Slide10
Entamoeba coli
Also with 2 stages: cyst and
trophozoite
.
Cyst: larger than
E.histolytica
, with 4-8 nuclei, if 4 there is no confusion with
E.histolytica
because it’s bigger and the
karyosome
is eccentric.
Trophozoite
: same
psuedopodia
shape but larger than
E.histolytica
, nuclei with eccentric
karyosome
, other
things
such as ingested particles or RBC’s and glycogen mass are not clear.Slide11
Entamoeba coli
Cyst
Trophozoite
Slide12Slide13
Endolimax nana
Very small in size, smaller than
E.histolytica
.
With 4 nuclei seen as a holes inside the cyst.Slide14
Endolimax nana Slide15
Iodamoeba
butschlii
Cyst small in size but larger than
E. nana
may be seen as an oval or ovoid shape have 1 large
nucleus
with glycogen mass, some time it may seen dark or as a hole with central large
karyosome
.Slide16
Iodamoeba
butschliiSlide17
Free living amoeba: AcanthamoebaSlide18
Naeglaria: