Protozoa Entamoeba histolytica Entamoeba histolytica A Trophozoites Irregular shape Transparent ectoplasm Granulated endoplasm small nucleus with perinuclear chromatin dots ID: 917214
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Slide1
Protozoa
Slide2Lab
12
Intestinal
Protozoa
Entamoeba
histolytica
Slide3Slide4Entamoeba
histolytica
A. TrophozoitesIrregular shape.Transparent ectoplasmGranulated endoplasm small nucleus with perinuclear chromatin dots, regular in size & spacing.
Slide5Trophozoites of
Entamoeba
histolytica
Slide6Slide71.
Entamoeba
histolytica b. cyst, note: round in shape outer cyst wall nuclei 1,2 or 4 chromatin bars may be present.
Slide8Cysts of
E.
histolytica
Cigar shape chromatoid body
Slide91.
Entamoeba
histolytica c. Section in infected intestine- note: Columnar epithelium, Lamina propria, Muscularis mucosae, Sub-mucosa, muscularis and serosa in intact mucosa
Clusters of amebae extending from epithelium
through lamina
propria
and
muscularis
mucosa and
muscularis
.
Some
amebae
show their nuclei and / or ingested
RBCs if the section passes through these structures.
Slide10Flask like ulcer caused by
E histolytica
Slide11ulcer of E.histolytica in large intestine
Fig 23-A
Fig 23-B
Slide12Slide13Hematophagous
’
trophozoites
Digested RBCs
Slide14Iodamoeba
butschlii A. Trophozoites nuclei with large central karyosome; karyosome surrounded
by small chromatin
granules;
Slide15Iodamoeba
butschlii B. cyst: large glycogen vacuole; nucleus as in trophozoites.
Slide16Iodamoeba
butschlii
Cyst ( 7 – 18 µm ) Iodamoeba butschlii Trophozoite31Iodamoeba butschlii from stool smear Iron hematoxylin stain
Iodine stain
Iodine stain
Slide17Intestinal
Protozoa
Commensal amoeba
Slide18Entamoeba
coli
A. Trophozoites: Irregular shape but more compact than E. histolytica;
Ectoplasm thin;
Granulate endoplasm;
Small
nucleus
with
perinuclear
chromatin
dots irregular in size &
spacing.
Slide19Entamoeba
coli
B. Cyst Large; nuclei 1,2,4 or 8;Chromatoid bars with pointed end.
Slide20Entamoeba
histolytica
Trophozoite( 18 – 60 µm )Entamoeba histolytica Cyst ( 5 – 20 µm ) Entamoeba Coli Cyst ( 15 – 33 µm )
Entamoeba Coli
Trophozoite
( 20 – 50 µm )
trophozoite
and cysts of
E
.
histolytica
and
E
. coli
form stool smear