histolytica and Entamoeba coli Asst Lec Iman KKadhim AlMustaqbal University college 2 st Class 2 st Semester Depart ID: 1040748
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1. parasitologyLab 2Entamoeba histolytica and Entamoeba coliAsst. Lec. Iman K.KadhimAl-Mustaqbal University college 2 st Class, 2 st Semester Department of pharmacy
2. Classification of parasitesdivided into three main groups:A –Protozoa single-celled organism, multiply in human host, All protozoans have 2 important stages of life: Trophozoite and Cyst1-phylum:Sarcomastigophoraa- subphylum: Sarcodinab-subPhylum:mastigophora2- Phylum: Ciliophora3- Phylum: Sporozoa B-Helminthes (worms) multicellular worms, do not normally multiply in human host1- Phylum: platyhelminthes الديدان المسطحه2- Phylum: Nematoda الديدان الخيطيه3- Phylum: Acanthocephala الديدان شوكية الراسC-Arthropoda multicellular worms, do not normally multiply in human host1- Phylum: insecta الحشرات2- Phylum: Archneida العناكب
3. Kingdom Animalia Subkingdom Protozoa Phylum Sarcomastigophora subphylum Sarcodina Class Lobosea Order Amoebida Family Endamoebidae Genus : Entamoeba Species : histolytica Kingdom Animalia Subkingdom Protozoa Phylum Sarcomastigophora subphylum Sarcodina Class Lobosea Order Amoebida Family Endamoebidae Genus : Entamoeba Species : coliEntamoeba coliEntamoeba histolytica Subkingdom: Protozoa; Phylum: Sarcomastigophora; subphylum Sarcodina
4. TrophozoitesEntamoeba coli Entamoeba histolytica15 mm - 40 mm in size 10 mm - 35 mm sizeMultiple pseudopodia Single pseudopodiaNon directional motility Unidirectional motilityNo ingested erythrocytes Ingested erythrocytes (RBC)Cytoplasm rough looking Finely granular cytoplasmLarge, eccentric karyosome Small, central karyosomeClumped nuclear chromatin Finely beaded chromatin
5. CystsEntamoeba coli Entamoeba histolytica10 mm - 35 mm in size 10 mm - 20 mm in sizeMay have 8 nuclei Never more than 4 nucleiKaryosomes eccentric Karyosomes small, centralNuclear chromatin clumped Chromatin finely beadedSplintered chromatoidal bars Rounded chromatoidal bars
6. Taxonomical Classification of Giardia lamblia Kingdom Protista Subkingdom Protozoa Phylum Sarcomastigophora Subphylum Mastigophora Class Zoomastigophora Order Diplomonadida Family Hexamitidae Genus Giardia Species lamblia Subkingdom: Protozoa; Phylum: Sarcomastigophora; Subphylum: Mastigophora
7. Giardia lamblia It is the most common flagellate of the intestinal tract. considered as one of the most common cause of infectious diarrhea throughout the world.Geographical Distribution: Worldwide (tropical and subtropical region)Humans are the only important reservoir of the infection. Causes Giardiasis, also called “traveler’s diarrhea” or “beaver fever”Morphology: exhibit the trophozoite and cyst .
8. Trophozoitepear or pyriform shapedfound in diarrheic stool rounded anteriorly and pointed posteriorlybilaterally symmetricallooking like tennis rackets without the handle Divide by binary fissionsucking disc (used for attachment of jejunal or duodenal mucosa)Motility by 4 pairs of flagellae 2 ventral and 2 caudalTwo oval nuclei with central karyosomeTwo axostyle traversing the bodyTwo rod-shaped parabasal bodies across the axostyle
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10. CystAverage size 12 X 7 µOval with well defined cyst wallFour nuclei present usually at one pole.Includes: axostyle – parabasal bodies – remnants of flagellaHabitat: duodenum and jejunumMature cyst is the infective stage
11. Life Cycle of Giardia inside human bodycysttrophozoiteEnter with foodPass in stoolBinary fissionDuodenal mucosa
12. Trichomonas vaginalisImportant features:A sexually transmitted disease (STD), Trichomonas vaginalis exists in only one morphological stage, a trophozoite. It is a pear-shaped organism with a central nucleus Four anterior flagella; and undulating membrane extends about two-thirds of its length
13. Human Trichomonads:3 species of trichomonads found in human.Two are normally harmless.Trichomonas hominis which inhabit large intestine & non pathogenic. Trichomonas tenax which inhabit oral cavity & commensals.Trichomonas vaginalis is the Urogenital pathogenic flagellate which is a serious sexually transmitted pathogen.
14. Causes : TrichomoniasisGeographical Distribution : worldwideHabitat: T. vaginalis trophozoite lives:In the vagina and urethra of infected females.In the urethra and Prostate of infected males(Never becomes cyst)Urogenital flagellate: Trichomonas vaginalisaxostyleflagellaNucleus
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16. TreatmentTo prevent reinfection, both partners should undergo treatment, and most common treatment for trichomoniasis is to swallow a single large dose of :1.Metronidazole (Flagyl) 2.Tinidazole (Tindamax) to reduce the risk of infection.