PPT-Identifying recombination events in phage Giles through presence of repeat sequences

Megan Mair Mycobacteriophage Giles Genes Unique to Giles Genes Conserved in Other Phages 72 57 genes 28 22 genes A B C D F G H I J K M N P R T Singleton clusters. Giles Corey’s personality. Very Stubborn old man. My personality could have been the fault of the accusation . I already been found guilty for murder of a farmer before. Some people . thought that . LOCK. it out on the first move. HI, DROXYLATE and . SWEET RELEASE defeat METHYLATE. but stay in play until cancelled. If host plays RESTRICT. then phage can counter with. METHYLATE – move both cards to the discard pile. Gil . McVean. There are no new questions in population genetics. …only new types of data. Genome sequences of entire populations. Data with linkage to deep phenotype information. Data from multiple species. MUTAGENESIS. DNA DAMAGE. DNA REPAIR. RECOMBINATION. MUTAGENESIS. Mutations. Mutations are heritable permanent changes in the base sequence of DNA.. Point mutations may be . transitions. (e.g. GC→AT) or . nanocrystals. doped with P, Li, S. N.V. . Derbeneva. , . V.A. . Burdov. Lobachevsky. State University of Nizhniy Novgorod. Published data . by other authors . on . radiative. recombination lifetimes. How to identify circularly permuted genomes, physical ends, 3’ overhangs, terminal repeats, and nicks.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. Lecture . 26. th. Alexei Fedorov, 2014. Origin of Chess. http://www.expert-chess-strategies.com/who-invented-chess.html. http://www.ponderabout.com/archives/972/mind-boggling-exponential-growth.aspx. Abdulrahman. . Alazemi. , . Shahroze. Abbas, Liam Lewis, Donald Ta, Ann Vo. Overview. Clustered repeats. Wide variety of functions. History largely unknown. Regulatory Sites. A segment capable of altering expression of specific genes. Lior . Tzabari. , Dan . Mendels, . Nir Tessler. Nanoelectronic center, EE Dept., Technion. Outline. Macroscopic View of recombination P3HT:PCBM. . - . Exciton Annihilation as the bimolecular loss. Drosophila . pseudoobscura. species group. Mohamed A. F. Noor. Duke University. EVOLUTION:. Two fundamental processes. Change within . lineages (adaptation important). Form new lineages . (speciation important). Crispr. /Cas9 cascade coding . cosmid. targeting . Stx. 1 and . Stx. 2 genes via lambda phage delivery system. Amanda Rutledge. How E. coli become Shiga toxin-producing. Escherichia coli. Shiga toxin-encoding phage. Pairs of allelic genes may exchange chromosomal locations( genetic elements can move, both between chromosomes and within them.) by a process known as genetic recombination.. Homologous recombination . or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. the attB1 & 2 sequences are relatively short and are therefore suitable for addition to gene-specific primers . After PCR amplification, fragment contains the attB1 and attb2 sequences, and can be used for recombination with a vector carrying the attP1 and atP2 sequences, and the addition of the BP clonase enzyme mix. .