Biol Res    Effect of alternating the magnetic eld on phosphate metabolism in the nervous system of Helix pomatia Ljiljana M Nikolic  Milos B Rokic  Natasa V Todorovic  Gordana S Kartelija  Miodrag S
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Biol Res Effect of alternating the magnetic eld on phosphate metabolism in the nervous system of Helix pomatia Ljiljana M Nikolic Milos B Rokic Natasa V Todorovic Gordana S Kartelija Miodrag S

bgacrs Received November 11 2009 In revised form March 16 2010 Accepted April 6 2010 17528721 Q57347UHFHQW57347GHFDGHV57347VFLHQWLILF57347LQWHUHVW57347LQ57347WKH HIIHFW57347RI57347VWDWLF57347DQG57347DOWHUQDWLQJ57347PDJQHWLF57347ILHOGV RQ57347WKH57347

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Biol Res Effect of alternating the magnetic eld on phosphate metabolism in the nervous system of Helix pomatia Ljiljana M Nikolic Milos B Rokic Natasa V Todorovic Gordana S Kartelija Miodrag S




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Biol Res 43: 243-250, 2010 Effect of alternating the magnetic field on phosphate metabolism in the nervous system of Helix pomatia Ljiljana M Nikolic , Milos B Rokic , Natasa V Todorovic , Gordana S Kartelija , Miodrag S Nedeljkovic and Joanna S Zakrzewska Department of Neurophysiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia, Institute for Multidisciplinary Studies, Kneza Viseslava 1, 11030 Belgrade, Serbia, Institute of General and Physical Chemistry, Studentski trg 12-16, 11000

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WXUQRYHUE\H[SRVXUHWRWKH(/)0)IRUWKHSHULRGRIGD\VDQGDGDSWDWLRQRISKRVSKDWHPHWDEROLVPLQWKH QHUYRXVV\VWHPRIVQDLOVWRSURORQJHG(/)0)H[SRVXUH (/)0)JDUGHQVQDLO 3105 VSHFWURVFRS\SKRVSKDWHWXUQRYHUHQ]\PHV Corresponding author: Ljiljana Nikoli , Research Assistant,

Department of Neurophysiology, Institute for Biological Research “Sinisa Stankovi ”, University of Belgrade Bulevar Despota Stefana 142, 11060 Belgrade, Serbia Tel: +381-11-2078-308 Fax: +381-11-2761-433 E-mail: ljnikolic@ibiss.bg.ac.rs Received: November 11, 2009. In revised form: March 16, 2010. Accepted: April 6, 2010. ,1752'8&7,21 ,QUHFHQWGHFDGHVVFLHQWLILFLQWHUHVWLQWKH HIIHFWRIVWDWLFDQGDOWHUQDWLQJPDJQHWLFILHOGV

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NIKOLI ET AL. BIOL RES 43, 2010, 243-250 247 Figure 2 31 P NMR spectrum of Helix pomatia isolated ganglion complex (n = 8). The signals are assigned to the following compounds: (1) Methylenediphosphonate (MDP, 17.05 ppm); (2) Phosphomonoesters (PME, 4.4 ppm); (3)

Inorganic phosphate (Pi, 2.6 ppm); (4) Phosphocreatine (PCr, -2.7 ppm); (5) -ATP (-4.7 ppm); (6) -ATP (-9.8 ppm); (7) -ATP (-18.4 ppm). Figure 3. A. 31 P NMR spectra of Helix pomatia ganglion complex for the control (n = 8), 7-day (n = 10), and 16- day (n = 10) ELF-MF (50 Hz, 0.5 mT) exposed groups of snails. The vertical line represents the chemical shift of the Pi signal. B. Intensity of 31 P NMR signals normalized to the intensity of standard (MDP) for the control, 7-day and 16-day ELF-MF exposed groups of snails. Error bars represent the absolute errors.

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indicates significant difference between 7 and 16-day ELF-MF exposed group of snails (one-way ANOVA, Fisher LSD test). Figure 5. Specific activity of acid phosphatase in the control (n = 5), 7-day (n = 3) and 16-day (n = 4) ELF- MF (50 Hz, 0.5 mT) exposed groups of snails, presented as mean  SEM. Note that the scaling factor on the y axis is 10 -7 . ***p < 0.001 indicates significant differences between control (7-day sham exposed) and 7-day ELF-MF exposed group of snails. < 0.05 indicates significant difference between 7 and 16-day ELF-MF exposed group of

snails (one-way ANOVA, Fisher LSD test).
Page 7
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