PDF-Mass Spectrometry

APPLICATION NOTE Author Avinash Dalmia PerkinElmer Inc Shelton CT USA Introduction Vanilla is the second most expensive spice and is widely used as a flavoring ingredient in the food beverage.  . Brent Fultz. , California Institute of . Technology. Department of Applied Physics and Materials Science . <. AP. . |. . h. . |. . MS. >. History. Recoil-Free Fraction. Technique, Samples. Protein Extraction. Mechanical grinding. Detergents. Other buffers. Sonication. http://www.piercenet.com/browse.cfm?fldID=FA97D803-6953-48E4-A7BD-6947D35FE83B. Considerations for mass spectrometry. Salts and buffers in high concentrations can cause ion suppression and adduct formation in electrospray mass spectrometry. Phil Charles. CCMP. Overview of Talk. Overview of proteomics as a concept. Techniques discussion. 2D Gels and experimental design paradigms. Proteomics mass spectrometry. Identification. Quantitation. Outline. Tandem Mass Spectrometry. De Novo Peptide Sequencing. Spectrum Graph . Protein Identification via Database Search. Identifying Post Translationally Modified Peptides . Spectral Convolution . Courtesy www.lab-initio.com. Purpose of Mass Spectrometry. P. roduces.  . spectra of masses from . the molecules . in . a sample of . material, and fragments of the molecules.. U. sed . to determine . Science Afternoon at NIST. February 4. th. , 2013. Questions Analytical (Bio)Chemists Ask. Environmental. Is the water safe to drink?. Deepwater. Horizon Oil Spill: Where did the oil go? When will the seafood be safe to eat again?. Introduction . to Spectroscopy. Spectroscopy involves an interaction between matter and light . (EMR). Light can be thought of as waves of energy or packets (particles) of energy called photons. Properties of light waves include wavelength and frequency. Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. ALONG WITH . H/D. EXCHANGE TO CHARACTERIZE . ANTIPLATELET DRUG DEGRADATION PATHWAYS.. A case report. .. B. ernard Do*, . Fatma . Amrani. , . Philippe-Henri . Secrétanand. . Najet. . Yagoubi. *Hospital Pharmacist . April 15. Bafna. Peptide MS. Instrument software usually detects peaks, and computes features (peak, area, m/z…). April 15. Bafna. m/z. Nobel Citation 2002. April 15. Bafna. Nobel Citation, 2002. April 15. Lecture 1. 4/13/2017. Announcements. Homework 2.2 Additional Problems - Due. Quiz Today (after Announcements). 4/18 Lecture – will go back to rest of NMR (interpretation examples and instrumentation), then continue on Mass . chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. Mass Spectrometry deals with the examination of characteristics fragments and arising from the breakdown of organic molecules. The mass spectrum is the plot of relative intensity of the ions agains