PPT-Recent applications of NGS sequencing in cancer studies

Andrew Gentles CCSB NGS workshop September 2012 Youve slogged through QC trimming alignment realignment variant calling What next Mutational processes molding the genomes of 21 breast cancersThe life history of 21 breast cancers. Credits for slides: Dan . Newburger. What is Cancer?. Definitions. A class of diseases characterized by malignant growth of a group of cells. Growth is uncontrolled. Invasive and Damaging. Often able to metastasize. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Eric T. Weimer, PhD, D(ABMLI). CONFLICT OF . INTEREST. I . have financial relationship(s) with: . Advisory Board. , Illumina. NGS Implementation at UNC. Received ASHI accreditation in . August . 2015.  . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Dr. Ronald Moura. ronaldmoura1989@gmail.com. https://www.linkedin.com/in/ronald-moura-660017178. /. Gordon Moore . “The . number of transistors in a dense integrated circuit doubles about every two . Biological Chemistry 3. DNA sequencing. Sequencing. 1) DNA sequencing: . Maxam. -Gilbert method. http://. en.wikipedia.org/wiki/Maxam%E2%80%93Gilbert_sequencing. 2) DNA sequencing: Sanger method. http://www.bio.davidson.edu/Courses/Bio111/seq.html. Vladimir Teif. Intro to NGS analysis. Proficio. course 2020. NGS techniques vs. NGS applications. NGS techniques. : . how. to sequence DNA (or RNA). (covered in lecture 1; funny recap in this video. for suspected cancer Information for patients and family members Genomic Medicine Service NHS What is your genome? Your genome is the information needed to build the human body and keep it health -- A Whirlwind tour. Presented by Daniel Winester, Geodesist, NGS Gravimetry Representative to the Geophysics Committee of U.S. National Section of PAIGH since 2002. In the past few decades NGS’ gravity work has concentrated on. 2. nd. August 2019. Sequencing history. Current sequencing approaches. File formats. Quality assessment. Read trimming. DAG workflows. The Sequence Read Archive. Outline. Descriptive text. Example commands – black monospaced text: . Newburger. What is Cancer?. Definitions. A class of diseases characterized by malignant growth of a group of cells. Growth is uncontrolled. Invasive and Damaging. Often able to metastasize. An instance of such a disease (a malignant tumor). - An overview of the technology. - Analysis software. - Fetal fraction (FF). - What is fetal fraction?. - Why fetal fraction is used. - Dynamic fetal fraction. Module 6:. NGS and Fetal . F. raction. in the Era of Precision Medicine. JSCO 50. th. Annual Meeting. Yokohama, Japan. October 27, 2012. Mark E. Sobel MD, PhD. Executive Officer. American Society for Investigative Pathology. mesobel@asip.org. ngs. ) of biopsy specimen. Molecular Cancer Classifier can Determine the Cancer Type, but Not Genetic Alterations in Cancer Cells. Comprehensive Profiling (NGS) Can Determine Genetic Alterations in Cancer Cells, but Can Not Determine the Cancer...