PPT-Fetal Growth Restriction

Conrad R Chao MD Professor of Obstetrics and Gynecology Chief of Maternal and Fetal Medicine University of New Mexico What is FGR SGA birthweight below 10 th percentile Associated with higher morbidity mortality and subsequent adult disease Barker hypothesis. Richard J. Roberts. New England Biolabs. CSHL - . 2013. AHA!. Danna, K., Nathans, D. (1971) Specific cleavage of simian virus 40 DNA by restriction endonuclease of . Hemophilus. influenza. . Proc. Natl. Acad. Sci. USA 68: 2913-2917. . Site-specific restriction endonucleases are used to identify DNA molecules. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . Assessment of Fetal Well Being Heather M. Said , DO Assistant Professor, Maternal Fetal Medicine Learning Objectives Discuss advantages and disadvantages of external versus internal fetal monitoring Be able to interpret fetal heart tracing Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Enzymes“. „. Sequence. . specific. . recognition. . and. . engineering. “. Alfred Pingoud. CSHL. Oct. . 19-21 2013. roberts@neb.com. . Sa 03.08.2013 . Outline of . talks. Alfred . Pingoud (25 . Effects of intrauterine retention and postmortem interval on body weight following intrauterine death: implications for assessment of fetal growth restriction at . autopsy. J Man, JC Hutchinson, M Ashworth, AE Heazell, S Levine and NJ Sebire . Dr . Soraya. . Saleh. . Gargari. Fellowship . feto. - maternal medicine. Shahid. . Beheshti. university. . At end of this lecture you should be able to:. . describe IUGR. . possible . etiologies. Venosus. Doppler. Insights from the Trial of Umbilical and Fetal Flow in . Europe. Tiziana FRUSCA, MD. 1*. ; Tullia TODROS, MD. 2*. , Christoph LEES, MD. 3,4. ; Caterina M. BILARDO, MD. 5. ; . and TRUFFLE Investigators. the isolation of the restriction . endonuclease. from. E. coli K (. Meselson. & Yuan 1968). . It was evident . that the restriction . endonucleases. from . E. coli K . and . E. coli B were important examples of proteins. Restriction Modification System. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. Vrushank. . Dharmesh. Bhatt. , Moussa . Diolombi. , Monika Haack, Janet . Mullington. Department . of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, . Boston, . MA . 02215. Choi Y, Jang W, Kim J, Ryu J, Lee S, Park K, et al. Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea. Emerg Infect Dis. 2005;11(2):237-244. https://doi.org/10.3201/eid1102.040603. Effectiveness and clinical application. Wyatt Kurzejeski, SPT. Learning objectives. 1. . The audience should be able to identify proper parameters for BFR use in a clinical setting, and explain the benefits and risks of this intervention.