PDF-Restriction Enzymes from Enzyme Finder

EnzymeCleavage Site 5 JBS EnzymeAatAGGGCCTAcc MKACAcc AccccCCGGAAccAGTTACTScaAccccGTACCKpnAfafaACRsa IA GWCCSin in TTAAGAgeeCCGGTAha ha AAA Dra Ahl hl CTAGTSpe AjnjnCCWGGAluluCTAlu IAlwlwTGCACApaAm. Chapter 2: Section 2.5. 1. Objectives. SWBAT explain the effect of a catalyst on activation energy. . SWBAT describe how enzymes regulate chemical reactions. . and maintain homeostasis.. 2. Starter: How can this be possible. Richard J. Roberts. New England Biolabs. CSHL - . 2013. AHA!. Danna, K., Nathans, D. (1971) Specific cleavage of simian virus 40 DNA by restriction endonuclease of . Hemophilus. influenza. . Proc. Natl. Acad. Sci. USA 68: 2913-2917. . Restriction Enzymes. General Genetics. Objectives:. Introduce the students to digest genomic DNA by restriction . endonucleases. .. Observe the results of digestion on . agarose. gel electrophoresis.. GENETIC. . ENGINEERING. Genetic engineering is the manipulation of genetic materials which can be introduced in the host organisms and thus change the phenotype of the host organism.. Protocol 10.1 through 10.3. Objective. : To cut phage genome into multiple fragments based on DNA sequence. General Introduction on Restriction Enzymes. Are also known as restriction endonucleases. Are naturally occurring enzymes used by bacteria for defensive purposes against extraneous DNA molecules. 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. are. . working. ?. Restriction. Enzymes. Natural . occurrence. . of. . restriction. . enzymes. . is. in . bacteria. . for. . protecting. . them. . against. a . virus. . attack. . by. . Sisters Enzyme . Video (5:47):. https://. www.youtube.com/watch?v=qgVFkRn8f10. Ricochet Science How Enzymes Work (. 1:20). https://. www.youtube.com/watch?v=UVeoXYJlBtI. AP Biology Catalase Lab Walkthrough (6:45). Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. DNA manipulating enzymes:. Restriction enzymes . Restriction enzymes . (or restriction endonucleases), are bacterial enzymes that cleave both strands of DNA at or near specific recognition nucleotide sequences (4 -8 bp ) known as restriction sites. . . biological . catalysts. . . . proteinaceous. . . specific . catalytic site . (active . centre. ). . some enzymes contain . . “. cofactor. ”( nonprotein organic compound) . . catalytically active. Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Steps in RDT:. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. Mechanical shearing. by. Prof. . Meena. . Nagawanshi. Professor . Department of . Zoology. Deogiri . College, Aurangabad. Enzymes. Enzymes are proteins. . biological catalysts .  help drive biochemical reactions. Enzyme names end with an .