PDF-J.-E.Shea,J.N.Onuchic,andC.L.Brooks,Proc.Natl.Acad.Sci.U.S.A.,12512Y.Q

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usingARTnouveauandparallelreplicashowthattheenergydifferencecanbeusedasagoodguidefortherespectiveweightatequilibriumIfweapplythiscriterionhereandsupposethattheentropyforbothmotifsareidenticaltherig

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J.-E.Shea,J.N.Onuchic,andC.L.Brooks,Proc.Natl.Acad.Sci.U.S.A.,12512Y.Q: Transcript

usingARTnouveauandparallelreplicashowthattheenergydifferencecanbeusedasagoodguidefortherespectiveweightatequilibriumIfweapplythiscriterionhereandsupposethattheentropyforbothmotifsareidenticaltherig. Proc.Natl.Acad.Sci.USA90(1993)8129Stimulusaz0C.)ww-JcnnNeuron'sResponseFourierFundamentalsAverage*Response=;;~~~~~~Noise=N-RiREALFIG.1.Calculationofaganglioncell'saverageresponseandresponsenoiseto10cy Proc.Natl.Acad.Sci.USA74(1977)4133.(iii)abFIG.5.Thepackingoftwoindependentpleatedsheets.(a)Twopleatedsheetsarerepresentedbytwothinsmoothsheets.Arrowsindicatethechaindirection.Eachsheethasaright-handed Proc.Natl.Acad.Sci.USA83(1986)3195U3R[U311PUvir5DNALTR-RUSU+ViralRNABamHIXhoILTRydLTPrototypePIli3400(3200)NheINheILEZ3EIILIZ3~MTZIJL*3200NT0,2200NT-SVNEO*3000NT*2200NTMT-NBLTRdLTRSV-N"1C7272U3NheIPvu Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist Proc.Natl.Acad.Sci.USA90(1993)solvedinapyrogenicwaterandthesolutionwasfilteredthrougha0.22-,mcelluloseacetatefilter.Thelaminarinfiltratewaslyophilizedandresuspendedtoaconcentrationof1.47mg/ml.Lipopoly Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea Proc.Natl.Acad.Sci.USA92(1995)7141FRETefficiencyRNAbulgeA7AgFIG.2.KinkingofDNAandRNAduplexesbybasebulgesisrevealedbyFRET.Thedistancebetweendonor(D)andacceptor(A)fluorophoresattachedtothe5'terminiofthe 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby 6130GeneticsLowryandZitomerdescribedbyShermanetal24Aftermutagenesiscellswereallowedtogrowfor-6generationsinYPDbeforeplatingMutantcolonieswereselectedforgrowthonCMlactateplatesMediaandGrowthConditionsS

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