PPT-Basics of high-throughput sequencing

Olivier Elemento PhD TA Jenny Giannopoulou PhD Institute for Computational Biomedicine CSHL High Throughput Data Analysis Workshop June 2012 Plan 1 What highthroughput sequencing is used for. Mayo/UIUC Summer . C. ourse in Computational Biology. Session Outline. Genome sequencing. Schematic overview of genome assembly. (a) DNA is collected from the biological sample and sequenced. (b) The output from the sequencer consists of many billions of short, unordered DNA fragments from random positions in the genome. (c) The short fragments are compared with each other to discover how they overlap. (d) The overlap relationships are captured in a large assembly graph shown as nodes representing . High-speed Networks. Esma. . Yildirim. Data Intensive Distributed Computing Laboratory. University at Buffalo (SUNY). Condor Week 2011 . Motivation. Data grows larger hence the need for speed to transfer it. Assembly, and Alignment Methods . Andy Nagar. Agenda. Background. Next Generation Sequencing. Sequence Assembly. Sequence Alignment. Traditional Alignment Algorithms. Next Generation Alignment Algorithms. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . November 11, 2011. 1. Ardavan Asef-Vaziri. Throughput, . Utilization, . Bottleneck, Effective Capacity, Theoretical Capacity, and Capacity Leverages.. Flow Rate, Throughput, . Takt. Time and Capacity. :. informatics & software aspects. Gabor T. Marth. Boston College Biology . Department. BI543 Fall 2013. January 29, 2013. Traditional DNA sequencing. Genetics of living organisms. DNA. Chromosomes. INTRODUCTION . The most commonly used technology until a few years ago – BAC. WHOLE GENOME SEQUENCING. ADVANTAGES OF WGS. Utility of next – gen sequence reads . The next-generation platforms are effecting a complete paradigm shift, not only in the organization of large-scale data production, but also in the downstream bioinformatics, IT, and LIMS support required for high data utility and correct interpretation.. 2b. Effective Capacity . &. Throughput Leverages. Based on the book: Managing Business Process Flows.. The . theoretical unit loads for an aggregate policy . (combination . of all policies) . in a . Minlan Yu. University of Southern California. 1. Joint work with . Wenjie. Jiang, . Haoyuan. Li, and Ion . Stoica. Throughput-Oriented Traffic. 2. Throughput-oriented traffic is growing in Internet. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. 1. The book Streamlined: 14 Principles for Building and Managing the Lean Supply Chain. 2004. . Srinivasan. . TOMPSON ISBN: 978-0-324-23277-6.. 2. The slides originally prepared by Professor M. M. . Srinivasan. Minlan Yu. University of Southern California. 1. Joint work with . Wenjie. Jiang, . Haoyuan. Li, and Ion . Stoica. Throughput-Oriented Traffic. 2. Throughput-oriented traffic is growing in Internet. Based on the books: . Building Lean Supply Chains with the Theory . of . Constraints . M. anaging . Business Process Flow . Throughput Profit Multiplier (TPM). A large fraction of the operating costs are fixed . Adeetya's Kitchen & Furniture in Pune offers a selection of top-quality kitchen trolleys to maximize storage space and improve the functionality of any kitchen. https://adeetyas.com/high-quality-kitchen-trolleys-in-pune.php