PPT-Basics of high-throughput sequencing

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Olivier Elemento PhD TA Jenny Giannopoulou PhD Institute for Computational Biomedicine CSHL High Throughput Data Analysis Workshop June 2012 Plan 1 What highthroughput

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Basics of high-throughput sequencing: Transcript

Olivier Elemento PhD TA Jenny Giannopoulou PhD Institute for Computational Biomedicine CSHL High Throughput Data Analysis Workshop June 2012 Plan 1 What highthroughput sequencing is used for. common use in biology. ese technologies produce millions of short sequence reads and are routinely being applied to genomes, epigenomes and transcriptomes. Sequencing steady-state RNA in a sample, microlens. array. Antony Orth and Kenneth . Crozier. 8 . May. CLEO . 2012. Microscopy with lens arrays. What is high . thoughput. microscopy?. Experimental setup – . confocal. system. Lens array characteristics, resolution. Microscope in the Big Data Era. TexPoint fonts used in EMF: . A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. Sreeram. . Kannan. and David Tse. Tutorial. ISIT 2014. Research supported by NSF Center for Science of Information.. :. informatics & software aspects. Gabor T. Marth. Boston College Biology . Department. BI543 Fall 2013. January 29, 2013. Traditional DNA sequencing. Genetics of living organisms. DNA. Chromosomes. Yao . Song. 11/05/2015. The rate of data to be processed for pattern matching increases rapidly.. network intrusion detection systems. Email monitoring systems. The content of data stream to be matched becomes very complex.. Fernández-Viña. Histocompatibility, . Immunogenetics. and Disease Profiling Laboratory. Stanford Blood Center. 2. Applications of NGS in Immunogenetics. HLA Typing. KIR Typing. Blood Group Typing. Harlan Robins. 1,2. Cindy Desmarais. 2. , Chris Carlson. 1,2. Fred . Hutchinson Cancer Research . Center, Seattle, WA. 1. , Adaptive . TCR. Technologies, Seattle, WA. 2. For additional information about . How cells are organized and work. Genetics: The Information. How the machinery is encoded in the genome and how the genome is inherited. Course Content. DNA sequencing and related approaches. Sample Preparation. . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Understanding New Tools from Federal Chemical Testing Programs . Linda Birnbaum. , Ph.D., D.A.B.T., A.T.S.. Director, National Institute of Environmental Health Sciences and National Toxicology Program. A CERN . openlab. / Intel collaboration. Niko Neufeld, CERN/PH-Department. niko.neufeld@cern.ch. HTCC in a nutshell. Apply upcoming Intel technologies in an Online / Trigger & DAQ context. Application domains: L1-trigger, data acquisition and event-building, accelerator-assisted processing for high-level trigger. TexPoint fonts used in EMF: . A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. Lecture 1. Instructor:. David Tse. dntse@stanford.edu. The Genome. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. Frontend. Dan Bradley. Center for High Throughput Computing. Condor Flocking. CHTC. Condor. Pool. CS. Nuclear Eng.. Genomics. CMS Tier 2. Atlas Tier 3. Chemical Engineering. IceCube. Medical. Physics. 1. M. Hammel, Y. Fan, A. Sarode, A.E. Byrnes, N. Zang, P. Kou, K. Nagapudi, D. Leung, C.C. Hoogenraad, T. Chen, C.-W. Yen, and G.L. Hura, . ACS Nano. . 17. , 11454 (2023), doi:10.1021/acsnano.3c01186. .

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