PPT-Purine and pyrimidine catabolism

Purine catabolism Purine degradation Purine Salvage Pathway From normal turnover of cellular nucleic acids Obtained from the diet Reutilization of adenine hypoxanthine and guanine Two enzymes. DNA: Picture 51. http://info.bio.cmu.edu/courses/03231/ProtStruc/ProtStruc.htm. B-DNA: The advent of modeling. http://molvis.sdsc.edu/pdb/dna_b_form.pdb. 46 Å. . 12 base sequence. (1953-2003). DNA: Size, Shape & Self Assembly. C483 Spring 2013. 1. A . ribose sugar is added to ________ rings after their synthesis and to ________ rings during their synthesis.. A) purine; pyrimidine. B) pyrimidine; purine. C) purine; purine. D) pyrimidine; pyrimidine. 1. Dr. Nikhat Siddiqi. 2. Dr. Nikhat Siddiqi. Biosynthesis of . histidine. in bacteria and plants. 3. Dr. Nikhat Siddiqi. Histidine. The pathway to . histidine. in all plants and bacteria . differs . Purines are biologically synthesized as nucleotides and in particular as ribotides, i.e. bases attached to ribose-5-phosphate. . Purines are synthesized by two types of pathways, the de novo pathways and the salvage pathways.. De Novo. Synthesis of . Purine. Nucleotides. We use for . purine. nucleotides the entire . glycine. molecule (atoms 4, 5,7), the amino nitrogen of . aspartate. (atom 1), amide nitrogen of glutamine (atoms 3, 9), components of the . OBJECTIVES:. Nomenclature of nucleic acids: . a. nucleosides*. . b. nucleotides. Structure . and function of . purines and pyrimidines. . Origin . of atoms in the purine . ring and in the pyrimidine ring.. Components of DNA. Sugar = Deoxyribose. Ribose is missing hydroxyl group (-. OH. ) from 2’C. Phosphate = PO. 4. 3-. Links to the sugar @ either its 3’C or its 5’C. Water (H. OH. ) is lost in the condensation reaction. Tail sheath. Tail fiber. DNA. 100 nm. 1. EXPERIMENT. Phage. DNA. Bacterial cell. Radioactive protein. Radioactive DNA. Batch 1: radioactive sulfur (. 35. S). Batch 2: radioactive phosphorus (. 32. P). One of the important specialized pathways of a number of amino acids is the synthesis of purine and pyrimidine nucleotides. . These nucleotides are important for a number of reasons. Most of them, not just ATP, are the sources of energy that drive most of our reactions.. OGY Dr. Sinosh Skariyachan, Department of Microbioogy, St. Pius X College Rajapuram, Kasaragod 1 DNA STRUCTURE Introduction - Historical Resume of Nucleic acid discovery  In 1869 Frederick Miesc Most cells actively turnover their. N.A.’s. especial RNA’s. This results in . the release . of adenosine, adenine, guanine. and . hypoxanthine. These are recovered. thru . salvage pathways. . The pathway consists of 6 enzymatic steps to form UMP. Unlike in . purine. biosynthesis. the . pyrimidine. ring is first formed and the the R5Pis added as PRPP. The N1,C4, 5 & 6 are all derived. nucleoside salvage. purine nucleoside ^. ribonucleoside ^. purine ribonucleoside ^. purine ribonucleoside salvage. urate *. purine ribonucleoside *. water-soluble vitamin *. nucleobase-containing small molecule ^. Prepared by. Harpreet Malhotra. Florida State College at Jacksonville. 24.1 Introduction (1). In the catabolism of . carbohydrates. , . glycolysis. converts . glucose. . into . pyruvate. , which is then metabolized into .