PPT-Purine and Pyrimidine anabolism

OBJECTIVES Nomenclature of nucleic acids a nucleosides b nucleotides Structure and function of purines and pyrimidines Origin of atoms in the purine ring and in the pyrimidine ring. DNA: Picture 51. http://info.bio.cmu.edu/courses/03231/ProtStruc/ProtStruc.htm. B-DNA: The advent of modeling. http://molvis.sdsc.edu/pdb/dna_b_form.pdb. 46 Å. . 12 base sequence. (1953-2003). DNA: Size, Shape & Self Assembly. OBJECTIVES:. Nomenclature of nucleic acids: . a. nucleosides*. . b. nucleotides. Structure . and function of . purines and pyrimidines. . Origin . of atoms in the purine . ring and in the pyrimidine ring.. C483 Spring 2013. 1. A . ribose sugar is added to ________ rings after their synthesis and to ________ rings during their synthesis.. A) purine; pyrimidine. B) pyrimidine; purine. C) purine; purine. D) pyrimidine; pyrimidine. Purine catabolism. Purine degradation. Purine Salvage Pathway. From normal turnover of cellular nucleic acids. Obtained from the diet. Reutilization of adenine, hypoxanthine, and guanine. Two enzymes:. M. etabolism. Nitrogenous Bases. Planar, aromatic, and heterocyclic. Derived from . purine. or . pyrimidine. Numbering of bases is “unprimed”. Nucleic Acid Bases. Purines. Pyrimidines. Sugars. Pentoses. Metabolism. Dr. Anissa Atif Mirza. Synopsis. Fates of dietary Nucleoproteins/Nucleic Acids.. De novo Biosynthesis of Purines and Pyrimidines.. Salvage of Purines and Pyrimidines. Catabolism of Purines and Pyrimidines. Tail sheath. Tail fiber. DNA. 100 nm. 1. EXPERIMENT. Phage. DNA. Bacterial cell. Radioactive protein. Radioactive DNA. Batch 1: radioactive sulfur (. 35. S). Batch 2: radioactive phosphorus (. 32. P). Chemistry of purines and pyrimidines. Nucleic Acid. Long chains of repeated nucleotides. A nucleotide consists of:. 1- Nitrogenous base. 2- Pentose sugar. 3- One or more phosphate groups. History. Nucleotide. life . processes. nucleotides . are the . activated precursors . of nucleic . acids. As such, they are necessary for the replication of the genome . an . adenine nucleotide, ATP, is . the universal currency of energy. A guanine . Acid. M.sc.. . Duaa. . falah. pharmacology and toxicology. Uric acid. It is . the final breakdown product of purine metabolism. it circulates in the plasma as sodium urate and is excreted by the kidney. It is derived from nucleic acid that are ingested or come from destruction of tissue cell .. OGY Dr. Sinosh Skariyachan, Department of Microbioogy, St. Pius X College Rajapuram, Kasaragod 1 DNA STRUCTURE Introduction - Historical Resume of Nucleic acid discovery  In 1869 Frederick Miesc nucleoside salvage. purine nucleoside ^. ribonucleoside ^. purine ribonucleoside ^. purine ribonucleoside salvage. urate *. purine ribonucleoside *. water-soluble vitamin *. nucleobase-containing small molecule ^. 4-methylthieno[2,3-. d. ]pyrimidine and their 4-oxo analogues. Introduction. . Drug discovery of . antibacterials. is in a challenging situation for decades [1,2] regardless the great number of the possible targets known [3] not many new antibiotics appear [4] and they are still from the same legacy classes [5]. In recent years the research in . Protein Synthesis. A histology technician studies the microscopic makeup of tissues, cells, and bodily fluids with the purpose of detecting and identifying the presence of a specific disease.. They determine blood types and the concentrations of drugs and other substances .