PPT-Object Groups & Enrichment Analysis

Suzanne Paley Pathway Tools Workshop 2010 What is an Object G roup A set of objects that are interesting for any reason eg Set of genes upregulated in an expression experiment Set of compounds in a pathway of interest or found in a particular cellular compartment. Analysis. :. Aaron Brooks. & . Fang Yin Lo. 8/21/2012. Introduction to Systems Biology 2012. making sense of big data. Mischel. et al, 2004. Experimental . Design. Data . collection. Data . analysis. A.thaliana. leaves. Robyn Klemptner. University of Johannesburg. MSc supervisors:. Dr. L.A. Piater. Prof. I.A. Dubery. Prof. R. Meijboom. Background. BIGGEST CHALLENGE. : . 9 BILLION people by 2050!!!. Anne Watson. NRich. July 2013. It’s not just methods .... Understanding: instrumental and relational (Skemp). Understanding: procedural and conceptual (Hiebert). Understanding: how to use procedures; when to use procedures; meaning of concepts (Watson). An engaging activity, hobby or past time where you can develop new skills and have fun learning. “High Expectations lead to High Achievers”. When is Enrichment? . It takes place every Wednesday afternoon from 2:00-3:30pm. Enrichment. You may sit wherever you want today.. If you brought supplies at Open House. , leave them on the window sill for now.. If you brought supplies with you today. , have them sitting on the desk in front of you.. rettigmd@jmu.edu. Professor Emeritus. James Madison University . Scheduling and Organizing the Intervention/Enrichment Period for Tiered Instruction in Response to Intervention. www.schoolschedulingassociates.com. The Dunlap Community will . empower . all student to . excel. in a global community. . . Becky Kuethe & Monica McComb. Our Focus Areas…. 1. Are we truly creating . CRITICAL THINKERS?. 2. Are we truly providing opportunities for . MSU. Echo . Wu, Ph.D. . 1. 2. Outline. What is Enrichment?. Process, Content & Product. Strategies for Enrichment. Within & Out-of-School Enrichment. What is Acceleration?. Strategies for Enrichment. N.C. Anheier, B.D. Cannon, C. Padilla-Cintron, . T. Pope, and C.A. Barrett. Pacific Northwest National Laboratory, USA. norm.anheier@pnnl.gov. PNNL is developing the Laser Ablation, Absorption Ratio Spectrometry (LAARS) method for destructive assay (DA) of uranium samples collected during enrichment plant inspections. . and RNA-. seq. Vladimir Teif. Intro to NGS analysis. Proficio. course 2020. NGS data integration. http://determinedtosee.com/wp-content/uploads/2014/08/jigsaw-puzzle.jpg. 1. Signal + existing annotation. simon.andrews@babraham.ac.uk. @. simon_andrews. v2022-02. What this course covers. The theory of . ChIP-Seq. ChIP-Seq. library properties. Sequencing, Data processing and QC. Data visualisation and exploration. Resources and Gene Set Enrichment Analysis. Genomics Lesson . 8_3. Hardison. resources. 3/24/15. 2. Try it yourself. Go to Gene Expression Omnibus at NCBI. http://. www.ncbi.nlm.nih.gov. /geo/. Choose a dataset of interest, e.g.:. 5-11 to 5-15-20. Enrichment 5-11 to 5-15-20. Edpuzzle. : . Enrichment 5-11: Negative Exponents. Edpuzzle. : . Enrichment 5-11: Squares and Square Roots. Edpuzzle. :. . Enrichment 5-11:Exponents and Square Roots. The PMN enrichment was carried out by double gradient centrifugation. A single cell suspension was (1x10^5 cells) stained with the following markers: CD15-BV421 (Pan-granulocyte), CD66b-FITC (Neutrophil maturity), CD16-APC (Neutrophil FC receptor), CCR3-APC-Cy7 (Eosinophil and basophil), and CD56-PerCP (NK cell). PMN population was gated based on the FSC-SSC scatter plot. The CD15+ and CD66b+ double positive cells were gated using a quadrant plot. This population indicated neutrophil enrichment, which was an average of 85.45% (± 6.20%). We also looked at contaminating eosinophil/basophil (CCR3) and NK-cell (CD56) populations by plotting against CD16 (neutrophil specific), and found them to be 1.14% (± 1.02%) and 1.9% (± 2.00 %) respectively. .