PPT-TRANSCRIPTION REVIEW

PROMOTER REGION REGION RNA POLYMERASE POLYMERASE WRITER S Phase Synthesis of DNA DNA REPLICATION DNA IS REPLICATED IN A SEMICONSERVATIVE MANNER In DNA Replication the entire genome doubles. and Its Regulation. January . 21 . –Mechanism of Transcription Initiation. January . 23– Regulation of of Transcription Initiation. January . 27–Mechanism and regulation of Transcription Elongation. Central Dogma of Biology. Information flows from . DNA .  RNA  Proteins. Think of it as exchanging money in a different country: proteins cannot be built directly from DNA. Three main processes involved:. Andrey Grigoriev. Director, Center for Computational and Integrative Biology. . Rutgers University. 2. What are we going to do?. Observe effects of fundamental processes. Estimate their relative contribution. Understanding:. Gene expression is regulated by proteins that bind to specific base sequences in DNA . The environment of a cell and of an organism has an impact on gene expression. Nucleosomes help to regulate transcription in eukaryotes. C483 Spring 2013. 1. RNA Polymerase. Polymerizes RNA in a 3’. 5’ direction.. Has 3’5’ . exonuclease. activity. Has 5’3’ . exonuclease. activity. Catalyzes a reaction driven by pyrophosphate hydrolysis. C483 Spring 2013. 1. Which . of the following is true about . transcription regulation? . A) A repressor protein activates transcription of a negatively regulated gene. . B) A positively regulated gene can be transcribed only in the absence of active repressor. . i. n Eukaryotes. Lecture #2. 1/23/17. References. . A few of the many insights from RNA polymerase structures. Cramer, P. (2002) . Multisubunit. RNA polymerases. . Curr. . Opin. . Struct. . Biol. BNFO 602/691. Biological Sequence Analysis. Mark Reimers, VIPBG. Why TFBS?. Protein transcription factors were among the first gene regulators identified. Many TFBS have distinct sequences, which were suitable for bioinformatics analysis. Structure. Central Dogma. Template Strand. Coding Strand. RNA Functions. RNA Building Blocks. Structure. Eukaryotic Process. RNA Polymerase. Subunits (Prokaryotes). α (2). β. β’. ω. σ. Transcription. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. the Lac Operon as an example. Dr. Vladimir Teif. BS222 – Genome Science Lecture 4. Module structure. Genomes, sequencing projects and genomic databases (VT) (Oct 9, 2018). Sequencing technologies (VT) (Oct 11, 2018). Genome architecture I: protein coding genes (VT) (Oct 16, 2018). . Dr. Shumaila Asim. Lecture # 2. 1. RNA Synthesis . (Transcription). . Introduction. First step in gene expression . RNA is involved in the transfer of genetic information stored in DNA to proteins . UserTo enable the closed captioningor live transcriptionfor usein your meetings1Sign in to the Zoom web portalhttps//zoomus2In the navigation panelon the left clickSettings3Click theMeetingtabThen cli Chargaff's rule. states DNA of any species contains equal amounts of guanine and cytosine and also equal amounts of adenine and thymine. . This pattern is found in both strands of the DNA. . They were discovered by Austrian chemist Erwin Chargaff.