PPT-Laying, Sequencing and Integration of r

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esilience programming Oct 2015 LAYERING IN REAL project Luuq Gedo Region SEQUENCING programming in SomReP State capacity building for resilience Community capacity

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Laying, Sequencing and Integration of r: Transcript

esilience programming Oct 2015 LAYERING IN REAL project Luuq Gedo Region SEQUENCING programming in SomReP State capacity building for resilience Community capacity building for resilience . January 2003www.inmongif.com LAYING LAYING LAYING LAYING HE HE HE HE TOVEPIPE TOVEPIPE TOVEPIPE TOVEPIPE RAGONRAGONRAGONRAGON . the. . table. Tablecloth. Table. . linen. – . it. . is. a . requisitive. part of . . catering. . facilities. and a . component. of . decoration. .. Tablecloth. . – an element of . Dan . Russell. Overview. Prologue: Assembly and Finishing. The Past: Sanger. The Present: Next-Gen (454, . Illumina. , …). The Future: ? (. Nanopore. , . MinION. , Single-molecule). Overview. Prologue: Assembly and Finishing. Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . 2 Timothy 1:6. “ . S. tir up . t. he gift of God which is in you through the laying on of my hands”. The need for strong foundations – Hebrews 6:1 – 2. Laying on of hands is seen in both the OT and NT. Making . Your Applications Virtual . Chris Green. Program Manager. Microsoft Corporation. SESSION CODE: . VIR320. Required Slide. Session Objectives And Takeaways. Understand the sequencing process. See best practices for . - . INTRODUCTION. - SANGER DIDEOXY METHOD. - AUTOMATED SEQUENCING. - NEXT. GENERATION OF SEQUENCING METHODS. MISS NUR SHALENA SOFIAN. INTRODUCTION. 1977:. . Frederick Sanger along with Allan . Maxam. - . INTRODUCTION. - SANGER DIDEOXY METHOD. - AUTOMATED SEQUENCING. - NEXT. GENERATION OF SEQUENCING METHODS. MISS NUR SHALENA SOFIAN. INTRODUCTION. 1977:. . Frederick Sanger along with Allan . Maxam. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. Hardison. Genomics 3_1. 1. 1/20/14. nucleo. s. ides. A nucleo. t. ide has one or more phosphates attached to the 5’ hydroxyl of the (deoxy)ribose. Building blocks of DNA. 1/20/14. 2. Structure of a dinucleotide. Adapted . from:. http. ://. ase.tufts.edu/chemistry/walt/sepa/geneticsofrace.html. Uploaded: January 8, 2017. Genetics of Race. Lesson 1: . Introduction. Goals:. Introduce module topic . Provide necessary background. The Sanger method. DNA sequencing is the primary method for gene and protein characterization. . (protein sequence can be predicted from DNA sequence). . https://. www.illumina.com. /techniques/sequencing/.

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