PPT-Ozone-induced changes in lung extracellular vesicle miRNA

Gregory J Smith PhD Assistant Professor Department of Genetics University of North Carolina at Chapel Hill Kelada Lab 1 Outline Lung EVs Ozone amp lung EV miRNA Future studies QampA. Introduction to Systems Biology Course. Chris . Plaisier. Institute for Systems Biology. Impetus for Tutorial. Searching for Amplified miRNAs. Region on chromosome 12 containing hsa-miR-26a is amplified in . Extracellular Vesicle. (PMID: 23420871). Extracellular Exosome . or “exosomes” (plural) . Formed in multivesicular bodies. ~40-200 nm. (PMID. : . 6151502 ,.  . 16487731, 15261674. , . 20601276). March 1. 7. , . 2010. Presented by . David B. Peden, M.D., M.S.. Professor of Pediatrics, Medicine & Microbiology/Immunology. Director, UNC Center for Environmental Medicine, Asthma and Lung Biology. mirVana™ miRNA Mimics miRNA Resuspension ProtocolWe recommend preparing 100 M miRNA stock solution. Dilute the stock Briey centrifuge the tube or plate to ensure that the dried miRNA is at Resu Emerging Clinical Applications. Mahmood rasheed. Hematology/oncology fellow. VCU Massey Cancer Center. Extracellular Vesicles. Heterogenous range of particles shed from virtually all cells in the human body.. A cell is the basic structural and functional unit of living organisms.. The activity of an organism depends on the collective activities of its cells.. Continuity of life has a cellular basis.. Chemical Components of Cells. brought to you by COREView metadata citation and similar papers at coreacukprovided by Diposit Digital de Documents de la UABPage 1of 26Early Sepsis Diagnosis via Protein and miRNA Biomarkers Point-of BackgroundChronic obstructive pulmonary disease COPD is a combination of small airway diseases like obstructive bronchiolitis and parenchymal destruction which causes chronic airow limitation It is o Setty. , M., . et al. Goal . Integrate multiple layers of data for tumor – DNA copy number, promoter methylation, mRNA expression, and . miRNA. expression.. Understand the role of . miRNA. -mediated and transcription factors (TFs) regulation. . Rikky. . Wenang. . Purbojati. miRNA. MicroRNA. (miRNA) is a class of RNA which is . believed to play important roles in gene regulation.. It’s a . short (21- to 23-nt) RNAs that bind to the 3′ . IN HUMAN FOLLICULAR FLUID. INTRODUCTION. . O. varian follicle is the. basic. functional unit of the ovary . containing. an oocyte surrounded by supporting granulosa and theca cells. . During follicle development, . Vermont Genetics Network. Annual Retreat. August 7, 2013. Hans Christian . Haverkamp. , Ph.D.. Johnson State College. Search Google for “Biomedical Research”. Research in the Exercise Physiology Lab. Harrison Cash MD. 1. , Andrew Timms PhD. 2. , . Ekram. Gad PhD. 3. , Nora . Disis. MD. 3. , Laura . Riolobos. PhD. 3. . University of Washington Medical Center, Department of Otolaryngology - Head & Neck Surgery, Seattle WA. Dr. . Berwyck. Poad. Compiled: 3 . February . 2017. Updated: 28 March 2017. Figure 1:. Overview of the experimental setup required to perform . OzID. in the ion-mobility spectrometry (IMS) cell of a Waters SYNAPT G2-Si. 2-20% ozone in oxygen (w/w) is generated externally and delivered to the IMS cell through a needle valve, yielding a pressure of ~3 .