PPT-Ozone-induced changes in lung extracellular vesicle miRNA

Gregory J Smith PhD Assistant Professor Department of Genetics University of North Carolina at Chapel Hill Kelada Lab 1 Outline Lung EVs Ozone amp lung EV miRNA Future studies QampA. Lee Jr Induced is a term implying that a particular condition is indirectly caused by another condition In the case of traffic volumes the term arose from the phenomenon that improvements to a highway especially capacity improvements seemed to res Introduction to Systems Biology Course. Chris . Plaisier. Institute for Systems Biology. Glioma. : . A Deadly Brain Cancer. Wikimedia commons. miRNAs in Cancer. Caldas et al., 2005. RISC. Utility of miRNAs in Cancer. computational . prediction and analysis. Resources. Lecture notes from previous years: Takis Benos and Ziv Bar-Joseph. Slides from: . www.bioalgorithms.info. Discovery of small RNAs. The first small RNA:. Introduction to Systems Biology Course. Chris . Plaisier. Institute for Systems Biology. Impetus for Tutorial. Searching for Amplified miRNAs. Region on chromosome 12 containing hsa-miR-26a is amplified in . Presented by: . Seyed. . Davar. . Siadat. (. Ph.D. ). Pasteur Institute of Iran. 1. WHAT ARE OMVs. ?. BASIC RESEARC!. 1- Small spherical structures 20–250 nm in diameter. . 2- Produced by growing cells, not products of cell . Extracellular Vesicle. (PMID: 23420871). Extracellular Exosome . or “exosomes” (plural) . Formed in multivesicular bodies. ~40-200 nm. (PMID. : . 6151502 ,.  . 16487731, 15261674. , . 20601276). March 1. 7. , . 2010. Presented by . David B. Peden, M.D., M.S.. Professor of Pediatrics, Medicine & Microbiology/Immunology. Director, UNC Center for Environmental Medicine, Asthma and Lung Biology. Emerging Clinical Applications. Mahmood rasheed. Hematology/oncology fellow. VCU Massey Cancer Center. Extracellular Vesicles. Heterogenous range of particles shed from virtually all cells in the human body.. prediction and analysis. Resources. Lecture notes from previous years: Takis Benos and Ziv Bar-Joseph. Slides from: . www.bioalgorithms.info. Discovery of small RNAs. The first small RNA:. In 1993 Rosalind Lee (Victor . . Asish Ghoshal. 1. , . Ananth. . Grama. 1. , . Saurabh. Bagchi. 2. , . Somali . Chaterji. 1. . Purdue University, West Lafayette, IN. . MicroRNA (miRNA): “The genome’s guiding hand?”. miRNA are 22 nucleotide . . WS 2019/20 - lecture 16. 1. Bioinformatics III. What are . microRNAs. ?. How can one . identify. microRNAs?. What is the . function. of microRNAs?. Laird, Hum Mol Gen 14, R65 (2005). Huntzinger, Izaurralde, Nat. Rev. Genet. 12, 99 (2011). The stage of meiotic prophase that immediately follows the zygotene. It is characterized by paired chromosomes that are condensed and visibly divided into chromatids, and by the occurrence of cr Harrison Cash MD. 1. , Andrew Timms PhD. 2. , . Ekram. Gad PhD. 3. , Nora . Disis. MD. 3. , Laura . Riolobos. PhD. 3. . University of Washington Medical Center, Department of Otolaryngology - Head & Neck Surgery, Seattle WA. Dr. . Berwyck. Poad. Compiled: 3 . February . 2017. Updated: 28 March 2017. Figure 1:. Overview of the experimental setup required to perform . OzID. in the ion-mobility spectrometry (IMS) cell of a Waters SYNAPT G2-Si. 2-20% ozone in oxygen (w/w) is generated externally and delivered to the IMS cell through a needle valve, yielding a pressure of ~3 .