PPT-Gene Expression: Transcription

part 2 Prepared by Prof Sabah Hassan Coordination by Prof Sabah Linjawi BIO 702 L 5 Eukaryotic mRNAs mRNA Production in Eukaryotes 1 Eukaryotic mRNAs have 3 main parts a The 5 leader sequence or 5 untranslated region 5 UTR varies in length. to the beginning of a transcriptional cycle. Vladimir P. Zhdanov*. PHYSICAL REVIEW E 80, 051925 (2009). Outline. Preliminary. Introduction. Model . of Gene . Transcription. Experimental Results. Conclusion. Learning Objectives. Learn what a ‘transcription factor’ is.. Learn how oestrogen affects gene transcription.. Learn what siRNA is and how it affects gene expression.. Recap of 15.1. That the cells in our bodies are highly . Outline. Objective. Mechanism. K. inase cascades and Phosphorylation/. dephosphorylation. . General. Learning/LTP. Synaptic strengthening. Control of gene expression. Transcription factors. Epigenetics. Evaluating Hap4’s Role in the Gene Regulatory Network that Controls the Response to Cold Shock in Saccharomyces cerevisiae using GRNmap K. Grace Johnson 1 , Margaret J. O’Neil 2 , Kam D. Dahlquist © 2017 W. H. Freeman and Company. CHAPTER 28 . Regulation of Gene Expression. DNA elements that control transcription. Protein factors that control transcription. Lac. operon as a model for regulation. TUMOR SUPRESSOR PROTEINS. ATP. ENZYMES THAT CONTROL CELLULAR RESPIRATION. ADAPTIVE GENE PRODUCTS ARE ONLY REQUIRED UNDER CERTAIN CONDITIONS. GENES FOR PROTEIN DIGESTION ARE TURNED OFF WHEN THERE IS NO PROTEIN AVAILABLE. . Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. (Chapter 9). Presented by Dr. Laurie M. Erickson. Chair of the Department of Health Sciences. Blitstein Institute of Hebrew Theological College. Chicago, IL, USA.. Gene Expression can be Regulated.. Gene expression: . . 1. The . trp. Operon. Contains 5 genes coding for proteins (enzymes) required for the synthesis of the amino acid tryptophan. Also contains a promoter and operator.. When tryptophan levels in the cell are low, the expression of the . Controlling gene expression is often accomplished by controlling transcription initiation.. Regulatory proteins . bind to DNA to either block or stimulate transcription, depending on how they interact with RNA polymerase.. Mechanism of regulation of gene expression- An overview. Gene activity is controlled first and foremost at the level of transcription.. Much of this control is achieved through the interplay between proteins that bind to specific DNA sequences and their DNA binding sites. . Mamoun Ahram, PhD. 1. Resources. This lecture. Cooper, Chapter 8. Regulation. of transcription in prokaryotes . The lac operon. 3. Metabolism of lactose. In the 1950s, pioneering experiments were carried out by François Jacob and Jacques Monod who studied regulation of gene transcription in E. coli by analyzing the expression of enzymes involved in the metabolism of lactose. . Acknowledgement. Centers for Disease Control and Prevention- Ethiopia (CDC-E). American Society for Clinical Pathology (ASCP). Addiss. Ababa University . University of Gondar. University of . Hawassa.