Fusion DNAreplicationAcentricfragment Dicentric Chromosome Formation Dicentricchromosome Radiationexposure Centromere Scoring Dicentrics with New Technologies 80 Slide Feeder Digital Camera and Image ID: 940319
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Breaks in two chromosomes Fusion DNAreplicationAcentricfragment Dicentric Chromosome Formation Dicentricchromosome Radiationexposure Centromere Scoring Dicentrics with New Technologies 80
Slide Feeder Digital Camera and Images Automated slide scanning stage. Automated Cytogenetic Workstation Digital images are used for scoring chromosomes aberrations (dicentrics). Scori
ng Dicentrics with New Technologies 80 Slide Feeder Digital Camera and Images Automated slide scanning stage. Automated Cytogenetic Workstation Digital images are used for scoring chromo
somes aberrations (dicentrics). Dicentric Chromosome Scoring Procedure 1. Write down the Cell IDlocated on the top of the slide.2. Count and record the number of centromeresto make sure
that the metaphase cell has 46 well spread chromosomes (range 45 3. Look for, count, and record dicentric chromosomeshave two primaryconstrictions (centromeres).
NormalDicentricOne X shaped structure Fusion of two X shaped structures = Centromere DicentricchromosomeAcentricfragment4. Look for, count and record acentri
c fragmentsfragments of DNA that do NOThave a centromere. 5. Look for, count and record centric ringsfragments of DNA in the shape of acircle with only a centromere pre
sent holding it together. Dicentric Chromosome Shape Centromere Centromeres in themiddle One centromereproximal and onein the center Both centromeresat the ends Chromosomes
are well separated without any overlap Overlap Centromeres are clearly visible with well separated chromosome arms Centromere(Primary constriction) Anaphase cells with completely se
parated sister chromatids mustbe rejected from analysis **Things to remember while scoring dicentric chromosomes** Occasionally, chromosomes with three centromeres (tricentric) and
four centromeres(quadricentric or tetra centric) are observed. A tricentric chromosome is scored as twodicentric chromosomes and a quadricentric chromosome is scored as threedicentric chrom
osomes. For example, if one dicentric chromosome and one tricentric chromosome are found in a metaphase cell then the total number of dicentric chromosomes observed is 3. Tricentric = 2 d
icentrics Quadricentric = 3 dicentrics Sample Chromosome Assays Sample #1 Sample #2 PairShare Dicentrics Activity:Work with a partner to determine the cell ID, number of centromeres, numbe
r of dicentric chromosomes, number of centric rings, and number of acentric fragments for each of the chromosome assays given in the shared Google slide presentation named PairShare Dicen
trics Activity”.Record all data in the table provided. Each student must turn in his or her own data sheet.Each chromosome assay is worth 10 points. Total = 100 points. Ticket Out t
he Door: 31 Activity **Turn in as you leave class today! List THREEobjects found within a chromosome assay. What are parts of a chromosome? Define ONEnew term used t