PDF-J.-E.Shea,J.N.Onuchic,andC.L.Brooks,Proc.Natl.Acad.Sci.U.S.A.,12512Y.Q

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usingARTnouveauandparallelreplicashowthattheenergydifferencecanbeusedasagoodguidefortherespectiveweightatequilibriumIfweapplythiscriterionhereandsupposethattheentropyforbothmotifsareidenticaltherig

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J.-E.Shea,J.N.Onuchic,andC.L.Brooks,Proc.Natl.Acad.Sci.U.S.A.,12512Y.Q: Transcript

usingARTnouveauandparallelreplicashowthattheenergydifferencecanbeusedasagoodguidefortherespectiveweightatequilibriumIfweapplythiscriterionhereandsupposethattheentropyforbothmotifsareidenticaltherig. usingARTnouveauandparallelreplicashowthattheenergydifferencecanbeusedasagoodguidefortherespectiveweightatequilibrium.Ifweapplythiscriterionhere,andsupposethattheentropyforbothmotifsareidentical,therig Proc.Natl.Acad.Sci.USA74(1977)4133.(iii)abFIG.5.Thepackingoftwoindependentpleatedsheets.(a)Twopleatedsheetsarerepresentedbytwothinsmoothsheets.Arrowsindicatethechaindirection.Eachsheethasaright-handed Proc.Natl.Acad.Sci.USA90(1993)424Age(months)200.0120z0.010laaCD-_-0.008oo100CQ0.006coCD~0o0.004x0NuclearDNATI0.002F0.04242a100000Q0la)co0x0Age(months)FIG.1.Steady-stateoxidativeDNAdamageincreaseswitha Proc.Natl.Acad.Sci.USA83(1986)3195U3R[U311PUvir5DNALTR-RUSU+ViralRNABamHIXhoILTRydLTPrototypePIli3400(3200)NheINheILEZ3EIILIZ3~MTZIJL*3200NT0,2200NT-SVNEO*3000NT*2200NTMT-NBLTRdLTRSV-N"1C7272U3NheIPvu Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist Proc.Natl.Acad.Sci.USA90(1993)solvedinapyrogenicwaterandthesolutionwasfilteredthrougha0.22-,mcelluloseacetatefilter.Thelaminarinfiltratewaslyophilizedandresuspendedtoaconcentrationof1.47mg/ml.Lipopoly Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA92(1995)inasignificantlyfasterreaction.Theformationrateofnativemoleculesonthisfastrefoldingchannelcorrespondstotherateexpectedforfoldingtooccurintheabsenceofpopulatedintermediate Proc.Natl.Acad.Sci.USA92(1995)FIG.1.CytologyofDrosophilafemalemeiosis.Theimagesareconfocalmicrographsofoocytesfixedandlabeledwithantibodiestovisualizemeioticstages.(A)MetaphaseIarrestinamaturestage14o 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby 2612Biochemistry:Sancaretal.PstIHaelilt*.JHinfl-TaqIadzJHpa11ti.r4HhaI'recAmRNA-recAprotein-Proc.Nati.Acad.Sci.USA77(1980)EcoRI-9A.-ii4------200020040060080010001200BasepairsFIG.1.Restrictionmapandseq 6130GeneticsLowryandZitomerdescribedbyShermanetal24Aftermutagenesiscellswereallowedtogrowfor-6generationsinYPDbeforeplatingMutantcolonieswereselectedforgrowthonCMlactateplatesMediaandGrowthConditionsS Proc-NatLAcadSciUSA9019938019co11rICSCNSMPIass-x0000Isi-2W4T-jINkIIIIIIIIII2NcP-oo0NPI000Nrs0aNooNoNNNCM0JwtNaICYiiID-iNccerco000os9zSzE3oSZlilEANwlMkIICCDON000oNVnoCD0cotNNNN--IICOCYwvrw5oIcasNecUo02

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