Part 2 Comparing Fluorescent Proteins 2013 Workshop C Cloning DNA to Make Proteins Dina N Kovarik MS PhD NWABR Fluorescent Proteins are Valuable Tools Locate proteins in the cell Track the migration of cells ID: 933599
Download Presentation The PPT/PDF document "Using BLAST to Analyze Proteins" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
Using BLAST to Analyze ProteinsPart 2: Comparing Fluorescent Proteins
2013 Workshop C: Cloning DNA to Make ProteinsDina N Kovarik, MS, PhDNWABR
Slide2Fluorescent Proteins are Valuable Tools
Locate proteins in the cellTrack the migration of cellsReporter of expression
Sister
centromeres
(green)
mark the attachment of microtubules
(red)
to sister chromatids (blue). Left: Normal. Right: Drug-treated.
Source: http://mct.aacrjournals.org/content/2/5.cover-expansion
Mice expressing GFP under UV light (left & right), compared to normal mouse (center). Source: Wikipedia.
Slide3Bioluminescence of the crystal jellyfish, Aequorea victoria
Source: http://www.conncoll.edu/ccacad/zimmer/GFP-ww/shimomura.html
238 amino acid proteins. GFP ribbon diagram. From PDB 1EMA.
Source: Wikipedia
Slide4Rainbow of Fluorescent Proteins
Source: Wikipedia. http://en.wikipedia.org/wiki/File:FPbeachTsien.jpg
“
D
rawn
” with bacteria
expressing 8 different fluorescent proteins
Diversity of Mutations
Diversity of Colors
“
mFruits
”
mBlueberry
(Blue Fluorescent Protein, or
BFP)
mLemon
(Yellow Fluorescent Protein, or
YFP)
mGrape1
(Cyan Fluorescent Protein, or
CFP)
Many
others, all with similarly ‘fruity’ names…
Slide5Diversity and Key Mutations
Slide6Research Questions
Is red fluorescent protein (RFP) related to its famous cousin, GFP, or is from a different source entirely? What other fluorescent proteins, if any, are closely related to RFP?
The cloning and protein purification experiments you have been conducting in the laboratory involve
mTomato
(related to
mCherry
), also called
red fluorescent protein (RFP).