Applications in Biomedicine Pooria Gill PhD of Nanobiotechnology pooriagillyahoocom In The Name of Allah Nucleic Acids DNA Linear Naturemade Genomic Cytoplasmic Manmade Oligo primer ID: 208294
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Nucleic Acids DHPLCApplications in Biomedicine
Pooria GillPhD of Nanobiotechnologypooriagill@yahoo.com
In The Name of AllahSlide2
Nucleic Acids
DNALinearNature-madeGenomicCytoplasmicMan-madeOligo-primer
Oligo
-probe
Q-DNA
Circular
Nature-made
Mitochondric
Organoplasmic
Man-made
Vectors
RNA
Nature-made
rRNA
mRNA
tRNA
ncRNA
tmRNA
Man-made
siRNASlide3
ApplicationsDHPLC Systems (e.g. WAVE Systems) can be used for a wide range of applications including:Mutation DetectionSizingOligonucleotide
Purification and AnalysisForensicsMicrobial AnalysisRNA Isolation and PurificationSingle Base ExtensionMethylationSlide4
With the WAVE System you can:Scan
fragments for both known and novel mutations/SNPs without extensive resequencingDetect low-abundance mutations in heterogeneous samplesEnrich for low-abundance allelesFractionate complex mixtures of related fragments based on differences in sequence content
Perform
targeted mutation/SNP scoring via primer extension assays
Analyze
end-point products from allele-specific
Probe/Primer-based Amplification Technologies Slide5
Probe/Primer-based Amplification TechnologiesPrimer-based Amplifications:Non-Isothermal Amplifications e.g. PCR-based MethodsIsothermal Amplifications/Non-PCR-based Amplifications:NASBA
HDASDA, and more than 45 N.A.I.A. TechnologiesProbe-based Amplifications:For example: Ligase Chain ReactionsSlide6
CARTRIDGE dependent Applications e.g. via WAVE Systems DNA-99-3510 DNASep Cartridge 4.6 mm x 50 mm, for variety of DNA applications including
Sizing and Mutation Detection, load capacity of 2µg DNA-99-3710 DNASep HT Cartridge 6.5 mm x 37 mm, for variety of DNA application but especially for Rapid Mutation Detection
, load capacity of 4µg
NUC-99-3550
OligoSep
® Cartridge 4.6 mm x 50 mm;
for
Oligo
Purification work only
NUC-99-3860
OligoSep
Prep HC Cartridge 7.8 mm x 50 mm,
for
Oligo
Purification
, load capacity of 5000µg
RNA-99-3810
RNASep
® Prep Cartridge 7.8 mm x 50 mm,
for variety of RNA applications
, load capacity of 50µg total RNA -OR- 1000ng of specific RNASlide7Slide8Slide9Slide10
Chromatograms and pedigree of patients 3 and 16. A. DHPLC analysis in normal control, patients 3 and 16. Both patients show abnormal DHPLC peak patterns with two peaks in comparison to normal control. B. Sequencing data in patient 3 and his family members. C. Sequencing data in patient 16 and his family members.Slide11
Example of Oligo Analysis by DHPLCSlide12Slide13
Thanks for Your Attentions
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