PPT-GAS CHROMATOGRAPHY Presented by

Dr Mrs C S Patil Professor amp Head Department of Chemistry VicePrincipal Deogiri College Aurangabad GAS CHROMATOGRAPHY GUIDED BY DR MRS CS PATIL MAM VICE PRINCIPAL OF DEOGIRI COLLEGE AURANGABAD. AP Investigation 5: Photosynthesis – Part I. Chromatography. T. echnique . used to separate a mixture into its component . molecules. The . molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper. Gas Chromatograph. Gas chromatography (GC). , is a common type of . chromatography . used in . analytical . chemistry for separating and analyzing compounds that can be vaporized without decomposition. . Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry GC . is currently one of the most popular methods for separating and analyzing compounds. . GC can be applied to the separation of any compound that is either naturally volatile (i.e., readily goes into the gas phase) or can be converted to a volatile derivative.. . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Dr . Debalina. . Basu. Department of Microbiology. Surendranath. College. Paper Chromatography. Principle. What is Descending Paper Chromatography?. Descending paper chromatography is an analytical technique in which the mobile phase moves downward through the stationary phase. In other words, in this method, the development of the paper occurs due to the movement of the solvent downwards on the paper. Therefore, the solvent reservoir should be at the top of the paper. In this process, the movement of the solvent is governed by gravity as well as capillary action.. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Introduction, theory, instrumentation, . derivatization. ,. Temperature programming, advantages, disadvantages and applications. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University.