PPT-Introduction to RNA-Seq & Transcriptome Analysis

Author : cady | Published Date : 2022-06-20

Jessica Holmes 1 PowerPoint by Shayan Tabe Bordbar Edited by Negin Valizadegan 2021 RNASeq Lab 2020 Introduction In this lab we will do the following On the IGB

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Introduction to RNA-Seq & Transcriptome Analysis: Transcript

Jessica Holmes 1 PowerPoint by Shayan Tabe Bordbar Edited by Negin Valizadegan 2021 RNASeq Lab 2020 Introduction In this lab we will do the following On the IGB Biocluster Use STAR . Next Generation Sequencer Applications. DeNovo Sequencing. Resequencing, Comparative Genomics. Global SNP Analysis. Gene Expression Analysis. Methylation Studies. ChIP . Sequencing-transcription factors, histones, polymerases. Unveiling . the Transcriptome using . RNA-. seq. Jinze. Liu. Outline. What is the transcriptome?. Measuring the transcriptome. Sampling the transcriptome using short reads. Alignment of reads to a reference genome. reconstruction and quantification. Lecture: algorithms & software solutions. Exercises II: de-novo assembly using . Trinity. Exercises I: read-mapping and quantification using . Cufflinks. Outline. With Emphasis on Resolution of the Perloidea. Pickett, BD. Shiozawa, DK. Whiting, MF. Davis, NG. Ridge, PG. Introduction. Data & Objectives. Data. Transcriptomes. 7 Plecoptera . – I’ll show you which ones. Systematic . In . silico. . analysis using public database. Sung Hwan Lee. 1,4. , Baek Gil . Kim. 2,3. , . Ho Kyoung Hwang. 1,4. , Woo . Jung . Lee. 1,4. , Chang . Moo Kang. 1,4**. . 1. Department . and . Data Analysis . Roy Williams . PhD . Sanford | Burnham Medical Research Institute . Microarray Revolution. Idea. : measure the amount of. . mRNA. . to see which. . genes. . are being. . expressed. Resources and Gene Set Enrichment Analysis. Genomics Lesson . 8_3. Hardison. resources. 3/24/15. 2. Try it yourself. Go to Gene Expression Omnibus at NCBI. http://. www.ncbi.nlm.nih.gov. /geo/. Choose a dataset of interest, e.g.:. Barbara Radovani. Genomics. . Master . of. Advanced . genetics. , UAB. Transcriptome. . Source. : https://www.genome.gov/13014330/transcriptome-fact-sheet/. The transcriptome, the entire repertoire of transcripts in. TexPoint fonts used in EMF: . A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. David Tse. Stanford University. Symposium on Turbo Codes and Iterative Information Processing . Bremen, Germany. August 20, 2014. Analysis. Jessica Holmes. High Performance Biological Computing (HPCBio). Roy J. Carver Biotechnology Center. General Outline. Getting the RNA-Seq data: from RNA -> Sequence data. Experimental and practical considerations. transcriptome. assembly and differential expression analysis. Ashley . Sawle. (. Ashley.Sawle@cruk.cam.ac.uk. ). Guillermo Parada (. guillermo.parada@sanger.ac.uk. ). HTS Applications - Overview. 2. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome.

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