PPT-Phage Nanobiotechnology

Tatiana Samoylova Phages ideal naturally occurring nanomaterials p hages viruses that infect bacteria exhibit a great diversity of shapes in nanometer range examples below. Geobacillus. and . Heamophilious. . Phages. Ramey . Elsarrag. Introduction. Tandem repeats are adjacently repeated patterns of DNA sequences. They are associated with genetic instability and are used for forensic identification. Khansa. . Bukhari. Maryum. . Zeb. . History and Definition. Frederick . Twort. (1915) and Felix . d'Herelle. (1917) were the first to recognize bacteriophage.. In the 1930s and after words virologists utilized these viruses as model systems to investigate many aspects of virology. Yvonne. . Lin. . AP. . Bio. . 2. a.k.a. Phages. Bacterial . VIRUS. Attacks, infects, and replicates within bacteria. Discovered. . by. . Frederick. . Twort. . (Great. . Britain, 1915). Species specific – named after the group or species it infects. Very complex shape, requiring 20 gene products for assembly. - . Capsid. (head), contains linear dsDNA genome. - . Tail. , consists of sheath plus internal tube. Bacteriophage T4. Figure 11.2. Phage T4 binds to . . discovery: potential applications for phage therapy . of . mycobacterial disease. Fernanda . Alonzo, . Adebanjo. . Adedoja. , . Ann . M. Findley, School of Sciences, . ULM; . Chris . R. . Gissendanner. . . Prepare phage for testing. *Create high titer lysate via flooding method.. *Isolate phage DNA and replicate using PCR.. *Send off isolated phage to HHMI facility for . Yang Xin. Paper presentation. PNAS 2009, 106(12):4629-4634. Introduction. Antimicrobial drug development is increasingly lagging behind the evolution of antibiotic resistance. as a result, there is a pressing need for new antibacterial therapies that can be readily designed. Series I: . Isolation and Purification of Phage. 1. We created an enrichment culture in order to grow bacteriophages and ultimately isolate a selected phage to examine. . 2. We generated a plaque assay using . How to identify circularly permuted genomes, physical ends, 3’ overhangs, terminal repeats, and nicks.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. Abdulrahman. . Alazemi. , . Shahroze. Abbas, Liam Lewis, Donald Ta, Ann Vo. Overview. Clustered repeats. Wide variety of functions. History largely unknown. Regulatory Sites. A segment capable of altering expression of specific genes. Index. Introduction. Discovery. Linkage data from transduction. Specialized transduction. Lytic and Lysogenic Cycle. Introduction. Transduction is the process by which DNA. . is transferred from one bacterium to another by a virus.. Nanobiotechnology. ?. The understanding and control of matter “. nano. ” meaning one-billionth – and new technologies that allow people to manipulate materials at the level of atoms and molecules.. . Josiah Austin, Julia Tang, Tiffany Nguyen, Michael . Ketteh. , Lynn Zhou, Dr. Mao PhD. The M13 phage is a human-friendly bacteriophage that only infects bacteria cells. 1. . It’s a nanofiber-shaped phage with five different coating proteins wrapped around its DNA (Figure 1b). The M13 phage can be engineered to display the exogenous peptides on its surface. This phage display technique allows us to study the function of short peptides that are derived from the cell’s growth factors, or proteins. Additionally, M13 phage have the ability to self-assemble into organized films that look like which can serve as a matrix for a cell culture. The phage film matrix can give us a better understanding of the role of short peptides in growth factors. The traditional method of making phage films requires the use of a pipette to drop the negatively charged phage solution onto the poly-lysine (Figure 2). However, manually, the results are not replicable and the process is time consuming. In order to make more organized and repeatable phage films we use a programmable slide stainer machine (Carl Zeiss, Inc.) that can dip the slides into a each solution allowing time to dry and giving it the ability to agitate. Resultantly, we built phage films while varying soaking time, phage concentration, and the number of phage layers to determine the best conditions to form well-ordered phage films with the help of slide stainer.. World’s Largest provider of commercial bacteriophage products for agricultural use.. Bacteriophage?. Bacteria-eater. Naturally occurring bacterial virus. Cannot infect mammalian, fungal, yeasts or molds.